pyrE

168

orotate phosphoribosyltransferase

Locus: BSU_15560

Molecular weight: 23.38 kDa

Isoelectric point: 5.63

Protein length: 216 aa

Gene length: 651 bp

Function: pyrimidine biosynthesis

Product: orotate phosphoribosyltransferase

Essential: no

E.C.: 2.4.2.10

Synonyms: pyrE, pyrX

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0461 (Galperin et al., 2021)

Gene

Coordinates: 1,629,320  1,629,970

The protein

Catalyzed reaction/ biological activity

• diphosphate + orotidine 5'-phosphate --> 5-phospho-α-D-ribose 1-diphosphate + orotate (according to UniProt)

Protein family

• Purine/pyrimidine phosphoribosyltransferase family (according to UniProt)

Structure

• 3DEZ (PDB) (from Streptococcus mutans, 59% identity, 74% similarity)
• P25972 (AlphaFold)

Expression and Regulation

Operons

• Genes: pyrR-pyrP-pyrB-pyrC-pyrAA-pyrAB-pyrK-pyrD-pyrF-pyrE

  Description: PubMed

  Regulation

  • induced in the absence of uridine nucleotides (PyrR) PubMed

  Regulatory mechanism

  • PyrR: termination, via RNA switch, in pyrR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE15560 (pyrE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAGATGTTTTGCGATGATTT,  downstream forward: _UP4_TAAAAAATAAATTCAAATGA
• BKK15560 (pyrE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAGATGTTTTGCGATGATTT,  downstream forward: _UP4_TAAAAAATAAATTCAAATGA

References

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