yhdR

168

similar to aspartate aminotransferase

Locus: BSU_09570

Molecular weight: 43.73 kDa

Isoelectric point: 5.01

Protein length: 393 aa

Gene length: 1182 bp

Function: unknown

Product: unknown

Essential: no

E.C.: 2.6.1.1

Synonyms: yhdR

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0436 (Galperin et al., 2021)

Gene

Coordinates: 1,034,046  1,035,227

The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + L-aspartate --> L-glutamate + oxaloacetate (according to UniProt), this activity is rather unlikely as the aspB mutant is auxotrophic for aspartate indicating that YhdR does not contribute to aspartate synthesis PubMed

Protein family

• class-I pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• PLP

Structure

• 3ELE (PDB) (from Eubacterium rectale, 34% identity)
• O07587 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• The gene is annotated in KEGG as aspartate aminotransferase EC 2.6.1.1. In MetaCyc the protein is marked as similar to aspartate aminotransferase. No EC annotation is available in Swiss-Prot. No literature/experimental evidence supporting the annotation is availablavailable.  PubMed

Expression and Regulation

Operons

• Genes: yhdR

  Description: PubMed

Biological materials

Mutant

• MGNA-B485 (yhdR::erm), available at the NBRP B. subtilis, Japan
• BKE09570 (yhdR::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTCCCTTCCGCATAT,  downstream forward: _UP4_TAAGAAGGCAAAGAAAAGAA
• BKK09570 (yhdR::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTCCCTTCCGCATAT,  downstream forward: _UP4_TAAGAAGGCAAAGAAAAGAA