ycgS

168

similar to aromatic hydrocarbon catabolism

Locus: BSU_03260

Molecular weight: 31.59 kDa

Isoelectric point: 8.23

Protein length: 284 aa

Gene length: 855 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ycgS

Genomic Context

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List of homologs in different organisms, belongs to COG0596 (Galperin et al., 2021)

Gene

Coordinates: 351,842  352,696

The protein

Protein family

• AB hydrolase superfamily (according to UniProt)

Domains

• AB hydrolase-1 domain (aa 25-123) (according to UniProt)

Structure

• 4Y7D (PDB) (from Nakamurella multipartita, 25% identity)
• P94396 (AlphaFold)

Expression and Regulation

Operons

• Genes: ycgS-ycgR-ycgQ

  Description: PubMed

Biological materials

Mutant

• MGNA-B997 (ycgS::erm), available at the NBRP B. subtilis, Japan
• BKE03260 (ycgS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTTTCACCCAATTGG,  downstream forward: _UP4_TAACCAAAGTGAAACGATCC
• BKK03260 (ycgS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCTGTTTCACCCAATTGG,  downstream forward: _UP4_TAACCAAAGTGAAACGATCC