Difference between revisions of "CymR"

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* '''Description:''' pleiotropic regulator of sulphur metabolism <br/><br/>
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* '''Description:''' pleiotropic regulator of [[sulfur metabolism]] <br/><br/>
  
 
{| align="right" border="1" cellpadding="2"  
 
{| align="right" border="1" cellpadding="2"  
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|style="background:#ABCDEF;" align="center"| '''Product''' || transcription repressor  
 
|style="background:#ABCDEF;" align="center"| '''Product''' || transcription repressor  
 
|-
 
|-
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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of sulphur metabolism
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|style="background:#ABCDEF;" align="center"|'''Function''' || regulation of [[sulfur metabolism]]
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Gene expression levels in [http://subtiwiki.uni-goettingen.de/apps/expression/ ''Subti''Express]''': [http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27520 cymR]
 +
|-
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|colspan="2" style="background:#FAF8CC;" align="center"| '''Interactions involving this protein in [http://subtiwiki.uni-goettingen.de/interact/ ''Subt''Interact]''': [http://subtiwiki.uni-goettingen.de/interact/index.php?protein=CymR CymR]
 +
|-
 +
|colspan="2" style="background:#FAF8CC;" align="center"| '''Metabolic function and regulation of this protein in [[SubtiPathways|''Subti''Pathways]]: <br/>[http://subtiwiki.uni-goettingen.de/subtipathways/search.php?enzyme=cymR cymR]'''
 
|-
 
|-
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 9.879   
 
|style="background:#ABCDEF;" align="center"| '''MW, pI''' || 11 kDa, 9.879   
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|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrvO]]'', ''[[yrvN]]''
 
|style="background:#ABCDEF;" align="center"|'''Immediate neighbours''' || ''[[yrvO]]'', ''[[yrvN]]''
 
|-
 
|-
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|colspan="2" style="background:#FAF8CC;" align="center"|'''Get the DNA and protein [http://srs.ebi.ac.uk/srsbin/cgi-bin/wgetz?-e+&#91;EMBLCDS:CAB14694&#93;+-newId sequences] <br/> (Barbe ''et al.'', 2009)'''
+
|style="background:#FAF8CC;" align="center"|'''Sequences'''||[http://bsubcyc.org/BSUB/sequence-aa?type=GENE&object=BSU27520 Protein] [http://bsubcyc.org/BSUB/sequence?type=GENE&object=BSU27520 DNA] [http://bsubcyc.org/BSUB/seq-selector?chromosome=CHROM-1&object=BSU27520 DNA_with_flanks]
 
|-
 
|-
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yrzC_context.gif]]
 
|colspan="2" | '''Genetic context''' <br/> [[Image:yrzC_context.gif]]
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 
  <div align="right"> <small>This image was kindly provided by [http://genolist.pasteur.fr/SubtiList/ SubtiList]</small></div>
 +
|-
 +
|colspan="2" |'''[http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cymR_2811717_2812133_-1 Expression at a glance]'''&#160;&#160;&#160;{{PubMed|22383849}}<br/>[[Image:cymR_expression.png|500px|link=http://subtiwiki.uni-goettingen.de/apps/expression/expression.php?search=BSU27520]]
 
|-
 
|-
 
|}
 
|}
  
 
__TOC__
 
__TOC__
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
<br/><br/><br/><br/>
 +
 +
 +
<br/><br/><br/><br/><br/>
  
−
<br/><br/>
+
= [[Categories]] containing this gene/protein =
 +
{{SubtiWiki category|[[sulfur metabolism]]}},
 +
{{SubtiWiki category|[[transcription factors and their control]]}},
 +
{{SubtiWiki category|[[regulators of core metabolism]]}}
  
 +
= This gene is a member of the following [[regulons]] =
 +
{{SubtiWiki regulon|[[stringent response]]}}
 +
=The [[CymR regulon]]=
 
=The gene=
 
=The gene=
  
 
=== Basic information ===
 
=== Basic information ===
  
−
* '''Coordinates:'''
+
* '''Locus tag:''' BSU27520
  
 
===Phenotypes of a mutant ===
 
===Phenotypes of a mutant ===
 +
* impaired growth in the presence of cystine and increased sensitivity to hydrogen peroxide-, disulfide-, paraquat- and tellurite-induced stresses {{PubMed|20608979}}
 +
* increased intracellular cysteine pool and hydrogen sulfide formation {{PubMed|20608979}}
 +
* depletion of branched-chain amino acids {{PubMed|20608979}}
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27520&redirect=T BSU27520]
  
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cymR-yrvO-trmU.html]
 
* '''DBTBS entry:''' [http://dbtbs.hgc.jp/COG/prom/cymR-yrvO-trmU.html]
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* '''Paralogous protein(s):'''
 
* '''Paralogous protein(s):'''
 +
 +
=== Genes repressed by CymR ===
 +
* ''[[ytmI]]-[[tcyJ]]-[[tcyK]]-[[tcyL]]-[[tcyM]]-[[tcyN]]-[[ytmO]]-[[ytnI]]-[[ytnJ]]-[[ribR]]-[[ytnL]]-[[ytnM]]'' {{PubMed|16109943}}
  
 
=== Extended information on the protein ===
 
=== Extended information on the protein ===
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* '''Effectors of protein activity:'''  
 
* '''Effectors of protein activity:'''  
  
−
* '''Interactions:''' [[CymR]]-[[CysK]], the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis [http://www.ncbi.nlm.nih.gov/sites/entrez/18974048 PubMed]
+
* '''[[SubtInteract|Interactions]]:'''
 +
** [[CymR]]-[[CysK]], the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis [http://www.ncbi.nlm.nih.gov/sites/entrez/18974048 PubMed]
  
−
* '''Localization:''' cytoplasm (according to Swiss-Prot)
+
* '''[[Localization]]:''' cytoplasm (according to Swiss-Prot)
  
 
=== Database entries ===
 
=== Database entries ===
 +
* '''BsubCyc:''' [http://bsubcyc.org/BSUB/NEW-IMAGE?type=NIL&object=BSU27520&redirect=T BSU27520]
  
−
* '''Structure:'''
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* '''Structure:''' [http://www.pdb.org/pdb/search/structidSearch.do?structureId=2Y75 2Y75] {{PubMed|21624051}}
  
−
* '''Swiss prot entry:'''
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* '''UniProt:''' [http://www.uniprot.org/uniprot/O34527 O34527]
  
−
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu+BSU27520]
+
* '''KEGG entry:''' [http://www.genome.jp/dbget-bin/www_bget?bsu:BSU27520]
  
 
* '''E.C. number:'''
 
* '''E.C. number:'''
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=Expression and regulation=
 
=Expression and regulation=
 +
* '''Operon:''' ''[[cymR]]-[[yrvO]]-[[trmU]]'' {{PubMed|16885442}}
  
−
* '''Operon:''' ''[[cymR]]-[[yrvO]]-[[trmU]]''
+
* '''Expression browser:''' [http://genome.jouy.inra.fr/cgi-bin/seb/viewdetail.py?id=cymR_2811717_2812133_-1 cymR] {{PubMed|22383849}}
  
−
* '''[[Sigma factor]]:''' [[SigA]]
+
* '''Sigma factor:''' [[SigA]] {{PubMed|16885442}}
  
 
* '''Regulation:'''  
 
* '''Regulation:'''  
 +
** [[RelA]] dependent downregulation (Class I) during stringent response {{PubMed|11948165}}
  
 
* '''Regulatory mechanism:'''  
 
* '''Regulatory mechanism:'''  
  
 
* '''Additional information:'''
 
* '''Additional information:'''
 +
** number of protein molecules per cell (minimal medium with glucose and ammonium): 508 {{PubMed|24696501}}
 +
** number of protein molecules per cell (complex medium with amino acids, without glucose): 1039 {{PubMed|24696501}}
  
 
=Biological materials =
 
=Biological materials =
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=Labs working on this gene/protein=
 
=Labs working on this gene/protein=
 +
 +
[[Isabelle Martin-Verstraete]], Institute Pasteur, Paris, France
  
 
=Your additional remarks=
 
=Your additional remarks=
  
 
=References=
 
=References=
 +
==The [[CymR regulon]]==
 +
<pubmed>16513748, </pubmed>
 +
==Other original publications==
 +
<pubmed>18974048,17056751,16109943,16885442 11948165 , 15668000 20608979 21624051</pubmed>
  
−
# Tanous et al. (2008) The CymR regulator in complex with the enzyme CysK controls cysteine metabolism in ''Bacillus subtilis''.''J. Biol. Chem.'' '''283:''' 35551-35560. [http://www.ncbi.nlm.nih.gov/sites/entrez/18974048 PubMed]
+
[[Category:Protein-coding genes]]
−
# Even S., Burguiere P., Auger S., Soutourina O., Danchin A., Martin-Verstraete I. (2006) Global control of cysteine metabolism by CymR in Bacillus subtilis. ''J. Bacteriol'' '''188:''' 2184-2197  [http://www.ncbi.nlm.nih.gov/sites/entrez/16513748 PubMed]
 
−
# Author1, Author2 & Author3 (year) Title ''Journal'' '''volume:''' page-page. [http://www.ncbi.nlm.nih.gov/sites/entrez/PMID PubMed]
 

Latest revision as of 09:35, 17 April 2014

Gene name cymR
Synonyms yrzC
Essential no
Product transcription repressor
Function regulation of sulfur metabolism
Gene expression levels in SubtiExpress: cymR
Interactions involving this protein in SubtInteract: CymR
Metabolic function and regulation of this protein in SubtiPathways:
cymR
MW, pI 11 kDa, 9.879
Gene length, protein length 333 bp, 111 aa
Immediate neighbours yrvO, yrvN
Sequences Protein DNA DNA_with_flanks
Genetic context
YrzC context.gif
This image was kindly provided by SubtiList
Expression at a glance   PubMed
CymR expression.png



























Categories containing this gene/protein

sulfur metabolism, transcription factors and their control, regulators of core metabolism

This gene is a member of the following regulons

stringent response

The CymR regulon

The gene

Basic information

  • Locus tag: BSU27520

Phenotypes of a mutant

  • impaired growth in the presence of cystine and increased sensitivity to hydrogen peroxide-, disulfide-, paraquat- and tellurite-induced stresses PubMed
  • increased intracellular cysteine pool and hydrogen sulfide formation PubMed
  • depletion of branched-chain amino acids PubMed

Database entries

  • DBTBS entry: [1]
  • SubtiList entry: [2]

Additional information

The protein

Basic information/ Evolution

  • Catalyzed reaction/ biological activity:
  • Protein family: Rrf2 family of transcription regulators
  • Paralogous protein(s):

Genes repressed by CymR

Extended information on the protein

  • Kinetic information:
  • Domains:
  • Modification:
  • Effectors of protein activity:
  • Interactions:
    • CymR-CysK, the complex is formed in the presence of cysteine, and results in DNA binding and repression of genes of cysteine biosynthesis PubMed

Database entries

  • KEGG entry: [3]
  • E.C. number:

Additional information

Expression and regulation

  • Regulation:
    • RelA dependent downregulation (Class I) during stringent response PubMed
  • Regulatory mechanism:
  • Additional information:
    • number of protein molecules per cell (minimal medium with glucose and ammonium): 508 PubMed
    • number of protein molecules per cell (complex medium with amino acids, without glucose): 1039 PubMed

Biological materials

  • Mutant:
  • Expression vector:
  • lacZ fusion:
  • GFP fusion:
  • two-hybrid system:
  • Antibody:

Labs working on this gene/protein

Isabelle Martin-Verstraete, Institute Pasteur, Paris, France

Your additional remarks

References

The CymR regulon

Sergine Even, Pierre Burguière, Sandrine Auger, Olga Soutourina, Antoine Danchin, Isabelle Martin-Verstraete
Global control of cysteine metabolism by CymR in Bacillus subtilis.
J Bacteriol: 2006, 188(6);2184-97
[PubMed:16513748] [WorldCat.org] [DOI] (P p)

Other original publications

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