ykoG

168

two-component response regulator (OmpR family)

Locus: BSU_13250

Molecular weight: 25.86 kDa

Isoelectric point: 5.11

Protein length: 228 aa

Gene length: 687 bp

Function: unknown

Product: two-component response regulator (OmpR family)

Essential: no

Synonyms: ykoG

Genomic Context

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List of homologs in different organisms, belongs to COG0745 (Galperin et al., 2021)

Gene

Coordinates: 1,391,953  1,392,639

The protein

Protein family

• OmpR family of two-component response regulators

Domains

• Response regulatory domain (aa 5-119) (according to UniProt)

Structure

• 5ED4 (PDB) (from Mycobacterium tuberculosis, 44% identity) PubMed
• O34903 (AlphaFold)

Modification

• phosphorylated by YkoH on an Asp residue

Effectors of protein activity

• phosphorylation likely affects DNA-binding activity

Paralogous protein(s)

• YclJ, WalR, PhoP

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: ykoG-ykoH-ykoI-ykoJ

  Description: PubMed

Biological materials

Mutant

• MGNA-A775 (ykoG::erm), available at the NBRP B. subtilis, Japan
• BKE13250 (ykoG::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATTTGACCATCCTCTCC,  downstream forward: _UP4_GGCTATGCCATTAAGGGGTA
• BKK13250 (ykoG::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAATTTGACCATCCTCTCC,  downstream forward: _UP4_GGCTATGCCATTAAGGGGTA

References

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