comGC

168

DNA uptake, major component of the pilus

Locus: BSU_24710

Molecular weight: 10.71 kDa

Isoelectric point: 6.32

Protein length: 98 aa

Gene length: 297 bp

Function: genetic competence

Product: major pilin

Essential: no

Synonyms: comGC

Genomic Context

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List of homologs in different organisms, belongs to COG4537 (Galperin et al., 2021)

Gene

Coordinates: 2,557,673  2,557,969

Phenotypes of a mutant

• knockout has complete loss of transformation competence PubMed

The protein

Catalyzed reaction/ biological activity

• captures extracellular DNA, pulls the bound DNA through the gap in the cell wall created during pilus biogenesis PubMed

Protein family

• comGC family (single member, according to UniProt)

Structure

• P25955 (AlphaFold)

Localization

• cell surface PubMed
• localizes to the cell membrane and the cytoplasm PubMed
• localizes to one single cell pole in the majority of the cells PubMed
• localizes along the long axis of competent cells PubMed

Expression and Regulation

Operons

• Genes: comGA-comGB-comGC-comGD-comGE-comGF-comGG-spoIIIL

  Description: PubMed

  Regulation

  • expressed early during sporulation in the forespore (SigF) PubMed

  Regulatory mechanism

  • ComK: activation, PubMed, in comK regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon
  • SigF: sigma factor, PubMed, in sigF regulon

  Additional information

  • the ''comGA-comGB-comGC-comGD-comGE-comGF-comGG-spoIIIL'' operon requires NusA for expression Reference

Biological materials

Mutant

• BKE24710 (comGC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCTCATTCATCAGCCTCT,  downstream forward: _UP4_ATCATCACCGGCGGAGAAGT
• BKK24710 (comGC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TTTCTCATTCATCAGCCTCT,  downstream forward: _UP4_ATCATCACCGGCGGAGAAGT
• BP914 ((comGA-comGB-comGC-comGD-comGE-comGF-comGG)::phleo), available in Fabian Commichau's lab

References

Reviews

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Original Publications

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