priA

168

primosomal replication factor Y, part of the replisome, required for survival of DNA damage

Locus: BSU_15710

Molecular weight: 91.16 kDa

Isoelectric point: 8.28

Protein length: 805 aa

Gene length: 2418 bp

Function: DNA replication initiation

Product: primosomal replication factor Y

Essential: yes

E.C.: 3.6.4.-

Synonyms: priA, yloJ

Genomic Context

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List of homologs in different organisms, belongs to COG1198 (Galperin et al., 2021)

Gene

Coordinates: 1,644,068 → 1,646,485

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• binds stalled replication forks and initiates fork restart allowing for chromosomes to be fully duplicated under stressful conditions PubMed
• binds to abasic DNA lesions PubMed

Protein family

• helicase family (according to UniProt)

Domains

• Helicase ATP-binding domain (aa 282-448) (according to UniProt)
• Helicase C-terminal domain (aa 545-699) (according to UniProt)

Structure

• 4NL4 (PDB) (from Klebsiella pneumoniae, 38% identity) PubMed
• P94461 (AlphaFold)

Expression and Regulation

Operons

• Genes: yloC-remA-gmk-yloH-coaBC-priA-defA-fmt-yloM-yloN

  Description

  • PubMed

Additional information

• the mRNA is cleaved by RNase III PubMed

References

Reviews

Carrasco B, Torres R, Moreno-Del Álamo M, Ramos C, Ayora S, Alonso JCProcessing of stalled replication forks in Bacillus subtilis.FEMS microbiology reviews. 2023 Dec 5; . PMID: 38052445
Michel B, Sandler SJ Replication Restart in Bacteria. Journal of bacteriology. 2017 Jul 01; 199(13). pii:e00102-17. doi:10.1128/JB.00102-17. PMID:28320884

Original Publications

Nischwitz E, Schoonenberg VAC, Fradera-Sola A, Dejung M, Vydzhak O, Levin M, Luke B, Butter F, Scheibe MDNA damage repair proteins across the Tree of Life.iScience. 2023 Jun 16; 26(6):106778. PMID: 37250769
Matthews LA, Simmons LAThe Bacillus subtilis PriA winged helix domain is critical for surviving DNA damage.Journal of bacteriology. 2022 Jan 10; :JB0053921. PMID: 35007156
Windgassen TA, Keck JL An aromatic-rich loop couples DNA binding and ATP hydrolysis in the PriA DNA helicase. Nucleic acids research. 2016 Nov 16; 44(20):9745-9757. . PMID:27484483
DiChiara JM, Liu B, Figaro S, Condon C, Bechhofer DH Mapping of internal monophosphate 5' ends of Bacillus subtilis messenger RNAs and ribosomal RNAs in wild-type and ribonuclease-mutant strains. Nucleic acids research. 2016 Apr 20; 44(7):3373-89. doi:10.1093/nar/gkw073. PMID:26883633
Bhattacharyya B, George NP, Thurmes TM, Zhou R, Jani N, Wessel SR, Sandler SJ, Ha T, Keck JL Structural mechanisms of PriA-mediated DNA replication restart. Proceedings of the National Academy of Sciences of the United States of America. 2014 Jan 28; 111(4):1373-8. doi:10.1073/pnas.1318001111. PMID:24379377
Sanders GM, Dallmann HG, McHenry CS Reconstitution of the B. subtilis replisome with 13 proteins including two distinct replicases. Molecular cell. 2010 Jan 29; 37(2):273-81. doi:10.1016/j.molcel.2009.12.025. PMID:20122408
Lecointe F, Sérèna C, Velten M, Costes A, McGovern S, Meile JC, Errington J, Ehrlich SD, Noirot P, Polard P Anticipating chromosomal replication fork arrest: SSB targets repair DNA helicases to active forks. The EMBO journal. 2007 Oct 03; 26(19):4239-51. . PMID:17853894
Marsin S, McGovern S, Ehrlich SD, Bruand C, Polard P Early steps of Bacillus subtilis primosome assembly. The Journal of biological chemistry. 2001 Dec 07; 276(49):45818-25. . PMID:11585815
Masai H, Deneke J, Furui Y, Tanaka T, Arai KI Escherichia coli and Bacillus subtilis PriA proteins essential for recombination-dependent DNA replication: involvement of ATPase/helicase activity of PriA for inducible stable DNA replication. Biochimie.; 81(8-9):847-57. . PMID:10572298