yitB

168

phospho-adenylylsulfate sulfotransferase

Locus: BSU_10930

Molecular weight: 27.52 kDa

Isoelectric point: 5.77

Protein length: 236 aa

Gene length: 711 bp

Function: sulfate reduction and activation

Product: phospho-adenylylsulfate sulfotransferase

Essential: no

E.C.: 1.8.4.8

Synonyms: yitB

Genomic Context

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List of homologs in different organisms, belongs to COG0175 (Galperin et al., 2021)

Gene

Coordinates: 1,171,755 → 1,172,465

The protein

Catalyzed reaction/ biological activity

• [thioredoxin]-disulfide + adenosine 3',5'-bisphosphate + 2 H⁺ + sulfite --> 3'-phosphoadenylyl sulfate + [thioredoxin]-dithiol (according to UniProt)

Protein family

• PAPS reductase family (with CysH, according to UniProt)

Structure

• 2OQ2 (PDB) (from yeast, 33% identity) PubMed
• O06737 (AlphaFold)

Paralogous protein(s)

• CysH

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yitB-yitA-yisZ

  Description: PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigK) PubMed

  Sigma factors

  • SigK: sigma factor, PubMed, in sigK regulon

Biological materials

Mutant

• MGNA-A504 (yitB::erm), available at the NBRP B. subtilis, Japan
• BKE10930 (ΔyitB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGTTTTCCCCCCGTTTG,  downstream forward: _UP4_TAGCGGATGGCAGGTTCTTG
• BKK10930 (ΔyitB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAATGTTTTCCCCCCGTTTG,  downstream forward: _UP4_TAGCGGATGGCAGGTTCTTG

References

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