yfkT

168

general stress protein, part of the YfkQ-YfkR-YfkT germinant receptor of unknown specificity

Locus: BSU_07760

Molecular weight: 40.20 kDa

Isoelectric point: 9.77

Protein length: 358 aa

Gene length: 1077 bp

Function: germination

Product: part of the YfkQ-YfkR-YfkT germinant receptor

Essential: no

Synonyms: yfkT

Genomic Context

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List of homologs in different organisms, belongs to COG5902 (Galperin et al., 2021)

Gene

Coordinates: 846,182  847,258

The protein

Protein family

• amino acid-polyamine-organocation (APC) superfamily (according to UniProt)
• Spore germination protein (SGP) (TC 2.A.3.9) family (according to UniProt)

Structure

• O34573 (AlphaFold)

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: yfkQ-yfkR-yfkS-yfkT

  Description: PubMed

  Regulation

  • expressed during sporulation in the forespore PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• MGNA-C343 (yfkT::erm), available at the NBRP B. subtilis, Japan
• BKE07760 (yfkT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAGCACATCCTTTCTG,  downstream forward: _UP4_TTGTTCGGCGCAAAAGCAAA
• BKK07760 (yfkT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATAAAGCACATCCTTTCTG,  downstream forward: _UP4_TTGTTCGGCGCAAAAGCAAA

References

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