cimH

168

transporter for citrate (proton symport)

Locus: BSU_38770

Molecular weight: 48.18 kDa

Isoelectric point: 6.55

Protein length: 450 aa

Gene length: 1353 bp

Function: citrate uptake

Product: transporter for citrate (proton symport)

Essential: no

Synonyms: cimH, yxkJ

Genomic Context

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List of homologs in different organisms, belongs to COG3493 (Galperin et al., 2021)

Gene

Coordinates: 3,979,753 → 3,981,105

The protein

Catalyzed reaction/ biological activity

• uptake of citrate (symport with protons) PubMed

Protein family

• sodium:citrate (SCF) symporter family (together with MaeN, according to UniProt)

Structure

• 5X9R (PDB) (from Klebsiella pneumoniae, 34% identity) PubMed
• P94363 (AlphaFold)

Paralogous protein(s)

• MaeN

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: cimH

  Description: PubMed

  Regulation

  • repressed by glucose (CcpA) PubMed

  Regulatory mechanism

  • CcpA: repression, PubMed, in ccpA regulon

  Additional information

  • the mRNA is substantially stabilized upon depletion of RNase Y PubMed

Biological materials

Mutant

• MGNA-B744 (yxkJ::erm), available at the NBRP B. subtilis, Japan
• BKE38770 (ΔcimH::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGAAAAACCTCCCCTAT,  downstream forward: _UP4_TGATCCCCGCAAGCAAGTTC
• BKK38770 (ΔcimH::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTGAAAAACCTCCCCTAT,  downstream forward: _UP4_TGATCCCCGCAAGCAAGTTC
• GP4216 (cimH::kan), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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