yqiI

168

secreted N-acetylmuramoyl-L-alanine amidase

Locus: BSU_24190

Molecular weight: 22.09 kDa

Isoelectric point: 4.29

Protein length: 206 aa

Gene length: 621 bp

Function: cell wall metabolism

Product: peptidoglycan hydrolase

Essential: no

Synonyms: yqiI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0860 (Galperin et al., 2021)

Gene

Coordinates: 2,514,979  2,515,599

The protein

Catalyzed reaction/ biological activity

• cell wall hydrolysis PubMed

Protein family

• N-acetylmuramoyl-L-alanine amidase 3 family (according to UniProt)

Domains

• contains an amidase_3 domain (like CwlC, CwlD, LytC, YrvJ)
• MurNAc-LAA domain (aa 32-201) (according to UniProt)

Structure

• 4RN7 (PDB) (from Clostridium difficile, 39% identity)
• P54525 (AlphaFold)

Localization

• secreted (with N-terminal signal sequence for SEC-mediated transport) PubMed

Expression and Regulation

Operons

• Genes: yqiH-yqiI-yqiK

  Description: PubMed

  Regulation

  • only expressed under conditions of high osmolarity PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-C372 (yqiI::erm), available at the NBRP B. subtilis, Japan
• BKE24190 (yqiI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTCAACTTACTCCCTC,  downstream forward: _UP4_TAGATTGATACAGCCTTAAT
• BKK24190 (yqiI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCTCAACTTACTCCCTC,  downstream forward: _UP4_TAGATTGATACAGCCTTAAT

References

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