aprX

168

intracellular alkaline serine protease

locus: BSU_17260

Molecular weight: 47.74 kDa

pI: 4.97

Protein length: 442 aa

Gene length: 1329 bp

function: protein degradation

product: intracellular alkaline serine protease

essential: no

synonyms: aprX

List of homologs in different organisms, belongs to COG1404 (Galperin et al., 2021)

Gene

Coordinates: 1,861,384  1,862,712

The protein

Protein family

• [wiki|peptidase S8 family] (according to UniProt)

[wiki|Domains]

• [wiki|Peptidase S8 domain] (aa 122-439) (according to UniProt)

Structure

• [PDB|3WHI] ([protein|0B98DE9CE2D98FFDE9F6EEB4E94FA1E5204BD48D|aprE], corresponds to aa 78 ... 436, 33% identity) [pubmed|24279884]
• [AF|O31788]

Expression and Regulation

Operons

• genes: [gene|945A1332A020CF842DA590589C88C758DE65C84E|aprX]

  description: [Pubmed|22383849]

  regulation

  • induced by DNA damage ([protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]) [Pubmed|16267290]

  regulatory mechanism

  • [protein|D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA]: repression, [Pubmed|16267290], in [regulon|protein:D267AF38B0CF107042326E9B8F3DD3C9C85840E4|lexA regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|10589719], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A019 (aprX::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/19 NBRP B. subtilis, Japan]
• BKE17260 ([gene|945A1332A020CF842DA590589C88C758DE65C84E|aprX]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTATTTTCCTCCTATTA,  downstream forward: _UP4_TAAACATCATCAAAAGCCGG
• BKK17260 ([gene|945A1332A020CF842DA590589C88C758DE65C84E|aprX]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17260 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATGTATTTTCCTCCTATTA,  downstream forward: _UP4_TAAACATCATCAAAAGCCGG

References

Reviews

• 34410368

Original Publications

• 10589719,16267290,24279884,38158017

Labs working on this gene/protein

• [wiki|Alessandra Albertini], University of Pavia, Italy [http://www-1.unipv.it/genbiom/albertini.html homepage]