ytpQ

168

putative alternative coproporphyrinogen III oxidase

locus: BSU_29830

Molecular weight: 30.89 kDa

pI: 5.3

Protein length: 269 aa

Gene length: 810 bp

function: biosynthesis of heme b under anaerobic conditions

product: putative alternative coproporphyrinogen III oxidase

essential: no

ec: null

synonyms: ytpQ

List of homologs in different organisms, belongs to COG4848 (Galperin et al., 2021)

Gene

Coordinates: 3,053,364  3,054,173

Phenotypes of a mutant

• derepression of the [protein|F899F1EE27E6D503BCC06BC52E3C7FD80B8EF725|fur] and [protein|00BCAAE16576DC5426A652580C69A570FC7A1C2C|perR] regulons [Pubmed|21949854]
• elevated oxidative protein damage upon exposure of the cells to methylglyoxal [Pubmed|21949854]
• the ''[gene|2C6386E9A63F410558D168798D077DF91590F454|spx] [gene|886EF2F8A9E031FDF919E01A858C1DBC1AFF1188|ytpQ]'' doulbe mutant shows reduced growth in minimal medium and hypersensitivity to methylglyoxal [Pubmed|21949854]

The protein

Catalyzed reaction/ biological activity

• [metabolite|coproporphyrinogen III] --> [metabolite|coproporphyrin III] [pubmed|38544863]

Protein family

• UPF0354 family (single member, according to UniProt)

Structure

• [AF|O34496]

Expression and Regulation

Operons

• genes: [gene|314D5C01B4BDDC3B122F6D4066647B143B2A6543|ytpP]-[gene|886EF2F8A9E031FDF919E01A858C1DBC1AFF1188|ytpQ]-[gene|EE559CD606562097F09DC627F431CA31129D7BC6|ytpR]

  description: [Pubmed|21949854]

  regulatory mechanism

  • [protein|2C6386E9A63F410558D168798D077DF91590F454|spx]: activation, [Pubmed|21949854], in [regulon|protein:2C6386E9A63F410558D168798D077DF91590F454|spx regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|21949854], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-A437 (ytpQ::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/437 NBRP B. subtilis, Japan]
• BKE29830 ([gene|886EF2F8A9E031FDF919E01A858C1DBC1AFF1188|ytpQ]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE29830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTCAAACCGATGTCCT,  downstream forward: _UP4_AAGGATTAGAAAGGATTTTT
• BKK29830 ([gene|886EF2F8A9E031FDF919E01A858C1DBC1AFF1188|ytpQ]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK29830 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATTTTCAAACCGATGTCCT,  downstream forward: _UP4_AAGGATTAGAAAGGATTTTT

References

• 21949854,38544863