yukD

168

Ubiquitin-like protein, part of the type VII protein secretion system YukD-EssB-YukB-YueB-YueC, putative bacteriocin

Locus: BSU_31900

Molecular weight: 8.94 kDa

Isoelectric point: 6.31

Protein length: 79 aa

Gene length: 240 bp

Function: export of YfjA, YukE, YxiB, YwqH

Product: ubiquitin-like protein, part of the type VII protein secretion system, putative bacteriocin

Essential: no

Synonyms: yukD

Genomic Context

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List of homologs in different organisms, belongs to COG5417 (Galperin et al., 2021)

Gene

Coordinates: 3,275,832  3,276,071

Phenotypes of a mutant

• loss of secretion of YukE PubMed

The protein

Catalyzed reaction/ biological activity

• export of YfjA, YukE, YxiB, YwqH PubMed
• secretion and delivery of LXG domain toxins PubMed

Protein family

• EsaB family (single member, according to UniProt)

Structure

• 2BPS (PDB)
• P71071 (AlphaFold)

Expression and Regulation

Operons

• Genes: yukE-yukD-essB-yukB-yueB-yueC-yueD

  Description: PubMed

  Regulation

  • expressed in the stationary phase PubMed
  • expression is reduced in motile cells as compared to non-motile cells PubMed

  Regulatory mechanism

  • DegU: activation, DegU-P, PubMed, in degU regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A627 (yukD::erm), available at the NBRP B. subtilis, Japan
• BKE31900 (yukD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTAGGATCACCCTTACA,  downstream forward: _UP4_ATATTATGAAAGGATCTGGT
• BKK31900 (yukD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTAGGATCACCCTTACA,  downstream forward: _UP4_ATATTATGAAAGGATCTGGT

References

Reviews

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Original Publications

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