rlmP

168

SPOUT methyltransferase, forms 2'-O-methylguanosine at position 2553 in the A-loop of 23S rRNA

Locus: BSU_28650

Molecular weight: 26.75 kDa

Isoelectric point: 5.18

Protein length: 248 aa

Gene length: 747 bp

Function: formation of 2'-O-methylguanosine at position 2553 in the A-loop of 23S rRNA

Product: SPOUT methyltransferase

Essential: no

E.C.: 2.1.1.-

Synonyms: ysgA

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0566 (Galperin et al., 2021)

Gene

Coordinates: 2,930,827  2,931,573

Phenotypes of a mutant

• essential PubMed, non-essential according to PubMed

The protein

Catalyzed reaction/ biological activity

• forms 2'-O-methylguanosine at position 2553 in the A-loop of 23S rRNA PubMed
• modifies free 23S rRNA but not the fully assembled ribosome nor the 50S subunit, suggesting that the modification occurs early during ribosome biogenesis PubMed

Protein family

• Methyltransferase superfamily (according to UniProt)
• class IV-like SAM-binding methyltransferase superfamily (with YacO and CspR, according to UniProt)

Cofactors

• SAM (according to UniProt)

Structure

• 4X3M (PDB)
• P94538 (AlphaFold)

Paralogous protein(s)

• YacO

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: rlmP

  Description: PubMed

Biological materials

Mutant

• MGNA-B006 (ysgA::erm), available at the NBRP B. subtilis, Japan
• BKE28650 (rlmP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGTCTATGTGGCGCTCC,  downstream forward: _UP4_TAGTGTTGCGCCAAGCGTTG
• BKK28650 (rlmP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CACTGTCTATGTGGCGCTCC,  downstream forward: _UP4_TAGTGTTGCGCCAAGCGTTG

References

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