ykfD

168

similar to oligopeptide ABC transporter (ATP-binding protein)

Locus: BSU_13000

Molecular weight: 36.83 kDa

Isoelectric point: 9.34

Protein length: 329 aa

Gene length: 990 bp

Function: uptake of dipeptides

Product: dipeptide ABC transporter (permease)

Essential: no

Synonyms: ykfD

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0444 (Galperin et al., 2021)

Gene

Coordinates: 1,368,844 → 1,369,833

The protein

Protein family

• ABC transporter superfamily (according to UniProt)

Domains

• ABC transporter domain (aa 7-252) (according to UniProt)

Structure

• 4FWI (PDB) (dipeptide transporter from Thermoanaerobacter tengcongensis, 36% identity) PubMed
• C0SP98 (AlphaFold)

Paralogous protein(s)

• OppF, AppF, AppD, DppD, OppD

Localization

• attached to the cell membrane (via DppB-DppC) PubMed

Expression and Regulation

Operons

• Genes: dppA-dppB-dppC-dppD-dppE-ykfA-ykfB-ykfC-ykfD

  Description: PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon

Biological materials

Mutant

• MGNA-A743 (ykfD::erm), available at the NBRP B. subtilis, Japan
• BKE13000 (ΔykfD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTCTCCCTCCTTATT,  downstream forward: _UP4_TAAATAAAAAGAGTGGCTCC
• BKK13000 (ΔykfD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGTCTCCCTCCTTATT,  downstream forward: _UP4_TAAATAAAAAGAGTGGCTCC

References

Loading