sacX

168

sucrose permease of the phosphotransferase system, EIIBC of the PTS, Trigger enzyme, control of SacY activity

Locus: BSU_38410

Molecular weight: 48.86 kDa

Isoelectric point: 9.35

Protein length: 459 aa

Gene length: 1380 bp

Function: sucrose uptake and phosphorylation, control of SacY activity

Product: sucrose-specific PTS permease, EIIBC component

Essential: no

E.C.: 2.7.1.69

Synonyms: sacX, ipa-14r, sacS

Genomic Context

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List of homologs in different organisms, belongs to COG1264 (Galperin et al., 2021)

Gene

Coordinates: 3,942,234  3,943,613

The protein

Protein family

• PTS permease, sucrose family PubMed

Domains

• PTS EIIB domain type-1 (aa 1-86) (according to UniProt)
• PTS EIIC domain type-1 (aa 106-459) (according to UniProt)

Structure

• P15400 (AlphaFold)

Paralogous protein(s)

• SacP, TreP, BglP, MurP

Localization

• cell membrane (according to UniProt)

Expression and Regulation

Operons

• Genes: sacX-sacY

  Description: PubMed

  Regulation

  • induction by sucrose (at high concentration) PubMed

  Regulatory mechanism

  • DegU: activation, DegU-P, PubMed, in degU regulon
  • SacY: antitermination, in sacY regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE38410 (sacX::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCAATTAAAACCTCCTT,  downstream forward: _UP4_TAACTGGATTTATTCGATTT
• BKK38410 (sacX::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CAAGCAATTAAAACCTCCTT,  downstream forward: _UP4_TAACTGGATTTATTCGATTT

References

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