ppsE

168

plipastatin synthetase

Locus: BSU_18300

Molecular weight: 144.39 kDa

Isoelectric point: 5.66

Protein length: 1279 aa

Gene length: 3840 bp

Function: production of the antibacterial compound plipastatin

Product: plipastatin synthetase

Essential: no

Synonyms: ppsE

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG3319 (Galperin et al., 2021)

Gene

Coordinates: 1,960,198 → 1,964,037

The protein

Protein family

• ATP-dependent AMP-binding enzyme family (according to UniProt)

Domains

• Carrier domain (aa 975-1050) (according to UniProt)

Structure

• 2VSQ (PDB) (SrfAC, 40% identity) PubMed
• O31827 (AlphaFold)

Localization

• cytoplasm (according to UniProt)

Expression and Regulation

Operons

• Genes: ppsA-ppsB-ppsC-ppsD-ppsE

  Description: PubMed

  Regulatory mechanism

  • AbrB: repression, PubMed, in abrB regulon

Biological materials

Mutant

• BKE18300 (ΔppsE::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTCTGCACCTTTCTTCACGG,  downstream forward: _UP4_TAAAGCGGATTAGCGGACAG
• BKK18300 (ΔppsE::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GTCTGCACCTTTCTTCACGG,  downstream forward: _UP4_TAAAGCGGATTAGCGGACAG

References

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