mtnW

168

2,3-diketo-5-methylthiopentyl-1-phosphate enolase, Rubisco-like protein

Locus: BSU_13590

Molecular weight: 44.89 kDa

Isoelectric point: 5.28

Protein length: 414 aa

Gene length: 1245 bp

Function: methionine salvage

Product: 2,3-diketo-5-methylthiopentyl-1-phosphate enolase

Essential: no

E.C.: null

Synonyms: mtnW, ykrW

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1850 (Galperin et al., 2021)

Gene

Coordinates: 1,427,034  1,428,278

The protein

Catalyzed reaction/ biological activity

• 5-methylsulfanyl-2,3-dioxopentyl phosphate --> 2-hydroxy-5-methylsulfanyl-3-oxopent-1-enyl phosphate (according to UniProt)

Protein family

• RuBisCO large chain family (single member, according to UniProt)

Structure

• 2ZVI (PDB) PubMed
• O31666 (AlphaFold)

Expression and Regulation

Operons

• Genes: mtnW-mtnX-mtnB-mtnD

  Description: PubMed

  Regulation

  • induced by methionine starvation (S-box) PubMed

  Regulatory mechanism

  • S-box: RNA switch, , the S-box riboswitch binds S-adenosylmethionine resulting in termination PubMed, in S-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE13590 (mtnW::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCAGAGCTCCTTTCAT,  downstream forward: _UP4_AAATGGGGAAAGGCTGAAGC
• BKK13590 (mtnW::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGCCAGAGCTCCTTTCAT,  downstream forward: _UP4_AAATGGGGAAAGGCTGAAGC

References

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