gsiB

168

general stress protein, prevents enzyme inactivation upon freeze-thaw treatments

Locus: BSU_04400

Molecular weight: 13.66 kDa

Isoelectric point: 5.14

Protein length: 123 aa

Gene length: 372 bp

Function: response to water deficits

Product: general stress protein

Essential: no

Synonyms: gsiB

Genomic Context

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List of homologs in different organisms, belongs to COG3729 (Galperin et al., 2021)

Gene

Coordinates: 494,506 → 494,877

The protein

Structure

• 3JQH (PDB) (human glycan binding receptor, 31% identity) PubMed
• P26907 (AlphaFold)

Expression and Regulation

Operons

• Genes: gsiB

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Sigma factors

  • SigI: sigma factor, PubMed, in sigI regulon
  • SigB: sigma factor, in sigB regulon

  Additional information

  • the mRNA is very stable (half-life > 15 min) PubMed

Biological materials

Mutant

• 1A735 ( gsiB::kan), PubMed, available at BGSC
• BKE04400 (ΔgsiB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTGAATTCCTCCTTTA,  downstream forward: _UP4_TAATCAAAAGCATAAAGGCA
• BKK04400 (ΔgsiB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTTGAATTCCTCCTTTA,  downstream forward: _UP4_TAATCAAAAGCATAAAGGCA

References

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