argD

168

acetylornithine transaminase

Locus: BSU_11220

Molecular weight: 40.74 kDa

Isoelectric point: 5.93

Protein length: 385 aa

Gene length: 1155 bp

Function: biosynthesis of arginine

Product: acetylornithine transaminase

Essential: no

E.C.: 2.6.1.11

Synonyms: argD

Genomic Context

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List of homologs in different organisms, belongs to COG4992 (Galperin et al., 2021)

Gene

Coordinates: 1,198,099 → 1,199,256

The protein

Catalyzed reaction/ biological activity

• 2-oxoglutarate + N²-acetyl-L-ornithine --> L-glutamate + N-acetyl-L-glutamate 5-semialdehyde (according to UniProt)

Protein family

• Class-III pyridoxal-phosphate-dependent aminotransferase family (according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 2EH6 (PDB) (from Aquifex aeolicus, 45% identity)
• P36839 (AlphaFold)

Paralogous protein(s)

• YhxA, RocD, GabT

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: argC-argJ-argB-argD-carA-carB-argF

  Description: PubMed

  Regulation

  • repressed by arginine (AhrC) PubMed
  • induced in the absence of RnpM PubMed

  Regulatory mechanism

  • AhrC: repression, PubMed, in ahrC regulon
  • CodY: repression, PubMed, in codY regulon
  • RnpM: unknown, PubMed, in rnpM regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE11220 (ΔargD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCTCATGAAACAGCCTCCTT,  downstream forward: _UP4_TGATTTTTTTTCGATATAAA
• BKK11220 (ΔargD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_GCTCATGAAACAGCCTCCTT,  downstream forward: _UP4_TGATTTTTTTTCGATATAAA

Expression vectors

• pGP3773: expression of Strep-argD by pGP380 in B. subtilis suitable for SPINE, available in Jörg Stülke's lab
• pGP3776: IPTG inducible expression, purification in E. coli with N-terminal Strep-tag, in pGP172, available in Jörg Stülke's lab
• pGP3792: IPTG inducible expression, purification in E. coli with N-terminal His-tag, in pWH844, available in Jörg Stülke's lab

References

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