yraF

168

forespore-specific sporulation protein, similar to spore coat protein

Locus: BSU_26960

Molecular weight: 13.87 kDa

Isoelectric point: 5.14

Protein length: 122 aa

Gene length: 369 bp

Function: unknown

Product: unknown

Essential: no

E.C.: null

Synonyms: yraF

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG5577 (Galperin et al., 2021)

Gene

Coordinates: 2,753,065  2,753,433

The protein

Protein family

• CotF family (according to UniProt)

Structure

• O07949 (AlphaFold)

Localization

• spore wall (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: yraG-yraF-adhB-yraE-yraD

  Description: PubMed

  Regulation

  • expressed during sporulation in the forespore (SigG) PubMed

  Sigma factors

  • SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

• MGNA-A028 (yraF::erm), available at the NBRP B. subtilis, Japan
• BKE26960 (yraF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTCAAAGAGGCTTACCTCC,  downstream forward: _UP4_CACTAACACTGGAGGTTTTA
• BKK26960 (yraF::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_ATTCAAAGAGGCTTACCTCC,  downstream forward: _UP4_CACTAACACTGGAGGTTTTA

References

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