nth

168

apurinic/apyrimidinic endonuclease , endonuclease III, removes abasic sites during base excision repair

Locus: BSU_22340

Molecular weight: 24.85 kDa

Isoelectric point: 9.28

Protein length: 219 aa

Gene length: 660 bp

Function: DNA repair

Product: endonuclease III

Essential: no

Synonyms: nth, jooB, ypoB

Genomic Context

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List of homologs in different organisms, belongs to COG0177 (Galperin et al., 2021)

Gene

Coordinates: 2,344,755 → 2,345,414

Phenotypes of a mutant

• the mutant is sensititive to exposure to H₂O₂ PubMed

The protein

Catalyzed reaction/ biological activity

• The C-O-P bond 3' to the apurinic or apyrimidinic site in DNA is broken by a beta-elimination reaction, leaving a 3'-terminal unsaturated sugar and a product with a terminal 5'-phosphate (according to UniProt)

Protein family

• Nth/MutY family (with MutY, according to UniProt)

Domains

• HhH domain (aa 109-128) (according to UniProt)

Cofactors

• Fe-S cluster PubMed

Structure

• 1P59 (PDB) (complex with DNA, Geobacillus stearothermophilus)
• P39788 (AlphaFold)

Effectors of protein activity

• activity is stimulated by untwisting of the DNA (by DnaD, Hbs, or YonN) PubMed

Expression and Regulation

Operons

• Genes: asnS-dnaD-nth-ypoC

  Description: PubMed

• Genes: dnaD-nth

  Description: PubMed

  Regulation

  • constitutively expressed PubMed

Biological materials

Mutant

• BKE22340 (Δnth::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAACACGATTGTCACCTTTT,  downstream forward: _UP4_GATAAAAAAGGACTGGTGAA
• BKK22340 (Δnth::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TAACACGATTGTCACCTTTT,  downstream forward: _UP4_GATAAAAAAGGACTGGTGAA

References

Reviews

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Original Publications

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