yfkC

168

mechanosensitive channel, similar to MscS

Locus: BSU_07940

Molecular weight: 32.05 kDa

Isoelectric point: 5.29

Protein length: 280 aa

Gene length: 843 bp

Function: resistance to osmotic downshock

Product: mechanosensitive channel, similar to MscS

Essential: no

Synonyms: yfkC

Genomic Context

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List of homologs in different organisms, belongs to COG0668 (Galperin et al., 2021)

Gene

Coordinates: 867,164  868,006

Phenotypes of a mutant

• a YfkC-R42W gain of function mutation facilitates the adaptation a strain lacking c-di-AMP to growth on medium containing glutamate PubMed

The protein

Catalyzed reaction/ biological activity

• the YfkC-R42W gain of function variant converts YfkC to a glutamate exporter (in conjunction with plsC, accA, or accC mutations that result in reduced phospholipid bisynthesis) PubMed

Protein family

• MscS (TC 1.A.23) family (according to UniProt)

Structure

• 3UDC (PDB) (from Caldanaerobacter subterraneus subsp. tengcongensis, 36% identity) PubMed
• O35043 (AlphaFold)

Localization

• cell membrane PubMed

Expression and Regulation

Operons

• Genes: yfkA-yfkC

  Description: PubMed

Biological materials

Mutant

• MGNA-C271 (yfkC::erm), available at the NBRP B. subtilis, Japan
• 1A959 ( yfkC::tet), PubMed, available at BGSC
• BKE07940 (yfkC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGTAAGTGTTTCTTTCATCC,  downstream forward: _UP4_TAAACAAAAAAACTGATTCC
• BKK07940 (yfkC::kan trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGTAAGTGTTTCTTTCATCC,  downstream forward: _UP4_TAAACAAAAAAACTGATTCC
• GP2785 (ΔyfkC::kan) , available in Jörg Stülke's lab
• GP3131 (yfkC-R42W kan plsC-L48P phleo) , available in Jörg Stülke's lab

References

Reviews

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Original Publications

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Labs working on this gene/protein

• Erhard Bremer, University of Marburg, Germany homepage