lytD

168

minor autolysin, peptidoglycan N-acetylglucosaminidase

Locus: BSU_35780

Molecular weight: 95.36 kDa

Isoelectric point: 9.94

Protein length: 880 aa

Gene length: 2643 bp

Function: minor autolysin, cell separation

Product: peptidoglycan N-acetylglucosaminidase

Essential: no

E.C.: 3.2.1.96

Synonyms: lytD, cwlG

Genomic Context

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List of homologs in different organisms, belongs to COG4193 (Galperin et al., 2021)

Gene

Coordinates: 3,684,826  3,687,468

The protein

Catalyzed reaction/ biological activity

• hydrolysis of the glycosidic bond between N-acetyl--d-glucosamine residues and adjacent monosaccharides PubMed
• Endohydrolysis of the N,N'-diacetylchitobiosyl unit in high-mannose glycopeptides and glycoproteins containing the -(Man(GlcNAc)(2))Asn-structure. One N-acetyl-D-glucosamine residue remains attached to the protein, the rest of the oligosaccharide is released intact (according to UniProt)

Protein family

• glycosyl hydrolase 73 family (with LytG, according to UniProt)

Domains

• 3 SPOR domains (aa 70-149, aa 150-229, aa 230-311) (according to UniProt)
• SH3B domain (aa 630-700) (according to UniProt)

Structure

• P39848 (AlphaFold)

Localization

• extracellular (signal peptide) PubMed

Expression and Regulation

Operons

• Genes: lytD

  Description: PubMed

  Sigma factors

  • SigD: sigma factor, PubMed, in sigD regulon

Biological materials

Mutant

• GP3400 (ΔlytD::neo trpC2), available in Jörg Stülke's lab
• 1A788 ( lytD::tet), PubMed, available at BGSC
• 1A792 ( lytD::tet), PubMed, available at BGSC
• BKE35780 (lytD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCTTCTCCTCTTTCTT,  downstream forward: _UP4_TAAAAAACTTAGAAAGTTGC
• BKK35780 (lytD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTCCTTCTCCTCTTTCTT,  downstream forward: _UP4_TAAAAAACTTAGAAAGTTGC

FLAG-tag construct

• GP4405 lytD-3xFLAG spc (based on pGP1331), available in Jörg Stülke's lab

References

Reviews

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Original Publications

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