pheT

168

phenylalanyl-tRNA synthetase (beta subunit)

Locus: BSU_28630

Molecular weight: 87.76 kDa

Isoelectric point: 4.86

Protein length: 804 aa

Gene length: 2415 bp

Function: translation

Product: phenylalanyl-tRNA synthetase (beta subunit)

Essential: yes

E.C.: 6.1.1.20

Synonyms: pheT

Genomic Context

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List of homologs in different organisms, belongs to COG0073 (Galperin et al., 2021)

Gene

Coordinates: 2,927,008 2,929,422

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• ATP + L-phenylalanine + tRNA^Phe --> AMP + diphosphate + H⁺ + L-phenylalanyl-tRNA^Phe (according to UniProt)

Protein family

• phenylalanyl-tRNA synthetase beta subunit family (single member, according to UniProt)

Domains

• tRNA-binding domain (aa 40-155) (accordinig to UniProt)
• B5 domain (aa 409-484) (according to UniProt)
• FDX-ACB domain (aa 710-803) (according to UniProt)

Structure

• 1PYS (PDB) (the PheS-PheT complex from Thermus thermophilus hb8 mutant, 37% identity, 56% similarity) PubMed
• P17922 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: pheS-pheT

  Description: PubMed

  Regulation

  • induced by phenylalanine limitation (T-box) PubMed
  • the T-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • T-box: RNA switch, via tRNA controlled RNA switch, in T-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

References

O'Reilly FJ, Graziadei A, Forbrig C, Bremenkamp R, Charles K, Lenz S, Elfmann C, Fischer L, Stülke J, Rappsilber JProtein complexes in cells by AI-assisted structural proteomics.Molecular systems biology. 2023 Feb 23; :e11544. PMID: 36815589
Gutiérrez-Preciado A, Henkin TM, Grundy FJ, Yanofsky C, Merino E Biochemical features and functional implications of the RNA-based T-box regulatory mechanism. Microbiology and molecular biology reviews : MMBR. 2009 Mar; 73(1):36-61. doi:10.1128/MMBR.00026-08. PMID:19258532
Eymann C, Dreisbach A, Albrecht D, Bernhardt J, Becher D, Gentner S, Tam le T, Büttner K, Buurman G, Scharf C, Venz S, Völker U, Hecker M A comprehensive proteome map of growing Bacillus subtilis cells. Proteomics. 2004 Oct; 4(10):2849-76. . PMID:15378759
Brakhage AA, Wozny M, Putzer H Structure and nucleotide sequence of the Bacillus subtilis phenylalanyl-tRNA synthetase genes. Biochimie. 1990 Oct; 72(10):725-34. . PMID:2127701