gerM

168

lipoprotein, germination (cortex hydrolysis) and sporulation, required for the assembly of the basal platform of the SpoIIIA-SpoIIQ trans-envelope complex during sporulation

Locus: BSU_28380

Molecular weight: 39.41 kDa

Isoelectric point: 4.68

Protein length: 366 aa

Gene length: 1101 bp

Function: germination (cortex hydrolysis) and sporulation

Product: SpoIIQ recruitment factor

Essential: no

Synonyms: gerM

Genomic Context

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List of homologs in different organisms, belongs to COG5401 (Galperin et al., 2021)

Gene

Coordinates: 2,902,002  2,903,102

Phenotypes of a mutant

• inactivation of ''gerM'' reduces sporulation efficiency to 0.3% that of wild type cells PubMed

The protein

Catalyzed reaction/ biological activity

• required for the assembly of the basal platform of the SpoIIIAH-SpoIIQ transenvelope complex during sporulation PubMed
• required for the recruitment of SpoIIQ to the septal membranes on the forespore side PubMed

Structure

• 6GZ8 (PDB) (first GerMN domain) PubMed
• 6GZB (PDB) (tandem GerMN domains) PubMed
• P39072 (AlphaFold)

Localization

• lipoprotein, outer forespore membrane, localization depends on SpoIIIAH and SpoIIQ PubMed

Expression and Regulation

Operons

• Genes: gerM

  Description: PubMed

  Regulation

  • expressed early during sporulation in the mother cell (SigE, SpoIIID) PubMed

  Regulatory mechanism

  • SpoIIID: repression, PubMed, in spoIIID regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon

Biological materials

Mutant

• BKE28380 (gerM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTTTTCCCTCCTAAGG,  downstream forward: _UP4_TAAATTGGGATTTTTGATAT
• BKK28380 (gerM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATACTTTTCCCTCCTAAGG,  downstream forward: _UP4_TAAATTGGGATTTTTGATAT

References

Reviews

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Original Publications

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