yfhK

168

general stress protein, similar to cell division inhibitor

Locus: BSU_08570

Molecular weight: 18.55 kDa

Isoelectric point: 9.96

Protein length: 172 aa

Gene length: 519 bp

Function: survival of ethonol stress and at low and high temperatures

Product: unknown

Essential: no

E.C.: null

Synonyms: yfhK

Genomic Context

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List of homologs in different organisms, belongs to COG3103 (Galperin et al., 2021)

Gene

Coordinates: 928,803  929,321

The protein

Domains

• 2 SH3B domains (aa 42-105, aa 112-172) (according to UniProt)

Structure

• O31579 (AlphaFold)

Expression and Regulation

Operons

• Genes: yfhK-yfhL-yfhM

  Description: PubMed

  Regulation

  • induced by stress (SigB) PubMed

  Sigma factors

  • SigB: sigma factor, PubMed, in sigB regulon
  • SigW: sigma factor, PubMed, in sigW regulon

Biological materials

Mutant

• MGNA-C317 (yfhK::erm), available at the NBRP B. subtilis, Japan
• BKE08570 (yfhK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAAAGCTCCTCCTCGC,  downstream forward: _UP4_TAAAAAGGCAGGCCGAGGCT
• BKK08570 (yfhK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAAAGCTCCTCCTCGC,  downstream forward: _UP4_TAAAAAGGCAGGCCGAGGCT

References

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