yhdA

168

NADPH-dependent FMN oxidoreductase

Locus: BSU_09340

Molecular weight: 18.76 kDa

Isoelectric point: 6.51

Protein length: 174 aa

Gene length: 525 bp

Function: protection against oxidative stress

Product: NADPH-dependent FMN oxidoreductase

Essential: no

Synonyms: yhdA, azr

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0431 (Galperin et al., 2021)

Gene

Coordinates: 1,010,445  1,010,969

The protein

Protein family

• azoreductase type 2 family (single member, according to UniProt)

Cofactors

• FMN PubMed
• NADPH PubMed

Structure

• 3GFQ (PDB) (99% identity),
• 1NNI (PDB)
• O07529 (AlphaFold)

Paralogous protein(s)

• PgcM, (31%)

Expression and Regulation

Operons

• Genes: yhcY-yhcZ-yhdA

  Description: PubMed

  Regulatory mechanism

  • LiaR: activation, PubMed, in liaR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-A689 (yhdA::erm), available at the NBRP B. subtilis, Japan
• BKE09340 (yhdA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGTGCCATTTATGACTAACA,  downstream forward: _UP4_GGCGTCTAAACGCCGGGATT
• BKK09340 (yhdA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CGTGCCATTTATGACTAACA,  downstream forward: _UP4_GGCGTCTAAACGCCGGGATT

References

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