panC

168

pantothenate synthetase

Locus: BSU_22420

Molecular weight: 31.81 kDa

Isoelectric point: 4.63

Protein length: 286 aa

Gene length: 861 bp

Function: biosynthesis of coenzyme A

Product: pantothenate synthetase

Essential: quasi

E.C.: 6.3.2.1

Synonyms: panC

Genomic Context

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List of homologs in different organisms, belongs to COG0414 (Galperin et al., 2021)

Gene

Coordinates: 2,352,977  2,353,837

Phenotypes of a mutant

• the mutant tends to acquire suppressor mutations that result in improved growth PubMed
• quasi-essential, the mutant is auxotrophic for pantothenate PubMed
• the mutant is viable if it acquires mutations (cymR or cysK) that allow constitutive expression of tcyJ-tcyK-tcyL-tcyM-tcyN for the uptake of cysteinopantetheine PubMed

The protein

Catalyzed reaction/ biological activity

• (R)-pantoate + ATP + beta-alanine --> (R)-pantothenate + AMP + diphosphate + H⁺ PubMed

Protein family

• pantothenate synthetase family (single member, according to UniProt)

Structure

• 2X3F (PDB) (from Staphylococcus aureus, 50% identity) PubMed
• P52998 (AlphaFold)

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: panB-panC-panD-dinG-ypmA-tseB-aspB

  Description: PubMed

Biological materials

Mutant

• BKE22420 (panC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGAAATATCAGTAATCTGTC,  downstream forward: _UP4_ATTCGAGAAATGGAGAGAAT
• BKK22420 (panC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGAAATATCAGTAATCTGTC,  downstream forward: _UP4_ATTCGAGAAATGGAGAGAAT
• GP4402 (panC::neo trpC2), available in Jörg Stülke's lab PubMed
• GP4361 (trpC2 ΔpanC::cat), available in Jörg Stülke's lab PubMed

Expression vectors

• pGP3723: expression of panC (gapA RBS) by pBQ200 in B. subtilis, available in Jörg Stülke's lab

References

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