ypsC

168

similar to SAM-dependent 23S rRNA methyltransferase

Locus: BSU_22170

Molecular weight: 43.35 kDa

Isoelectric point: 8.01

Protein length: 385 aa

Gene length: 1158 bp

Function: rRNA modification

Product: 23S rRNA methyltransferase

Essential: no

Synonyms: ypsC

Genomic Context

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Categories containing this gene/protein

Information processing, Protein synthesis, modification and degradation, Translation, rRNA modification and maturation/ based on similarity

List of homologs in different organisms, belongs to COG0116 (Galperin et al., 2021)

Gene

Coordinates: 2,330,075  2,331,232

The protein

Protein family

Methyltransferase superfamily (according to UniProt)

Domains

THUMP domain (aa 44-156) (according to UniProt)

Structure

3K0B (PDB) (from Listeria monocytogenes, 63% identity)
P50840 (AlphaFold)

Expression and Regulation

Operons

Genes: gpsB-rnpB-ypsC

Description: PubMed

Genes: rnpB-ypsC

Description: PubMed

Biological materials

Mutant

MGNA-A416 (ypsC::erm), available at the NBRP B. subtilis, Japan
BKE22170 (ypsC::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTATCACCCTGTCTTT,  downstream forward: _UP4_TAAAAAGAAGACGCTCTGCA
BKK22170 (ypsC::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTATCACCCTGTCTTT,  downstream forward: _UP4_TAAAAAGAAGACGCTCTGCA

Two-hybrid system

B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Fabian Commichau's lab

References

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