yplP

168

transcriptional activator (for SigL-dependent promoter)

Locus: BSU_21780

Molecular weight: 37.05 kDa

Isoelectric point: 9.3

Protein length: 331 aa

Gene length: 996 bp

Function: required for survival at low temperatures

Product: transcriptional activator (for SigL-dependent promoter)

Essential: no

Synonyms: yplP

Genomic Context

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List of homologs in different organisms, belongs to COG1221 (Galperin et al., 2021)

Gene

Coordinates: 2,294,286  2,295,281

Phenotypes of a mutant

• The mutant is cold-sensitive [http://www.ncbi.nlm.nih.gov/sites/entrez/16585774  PubMed]

The protein

Protein family

• Transcription factors activating transcription at SigL-dependent promoters

Domains

• Sigma-54 factor interaction domain (aa 12-213) (according to UniProt)

Structure

• 3DZD (PDB) (Aquifex aeolicus NtrC, corresponds to aa 12 ... 333 of YplP, 33% identity) PubMed
• P54156 (AlphaFold)

Expression and Regulation

Operons

• Genes: yplP-trhA

  Description: PubMed

Biological materials

Mutant

• MGNA-A896 (yplP::erm), available at the NBRP B. subtilis, Japan
• GP2304 ( yplP::aphA3), available in Jörg Stülke's lab
• BKE21780 (yplP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAATTGGCTCCTTTTGG,  downstream forward: _UP4_TAGCGATCGAAAGGAGAATG
• BKK21780 (yplP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCAATTGGCTCCTTTTGG,  downstream forward: _UP4_TAGCGATCGAAAGGAGAATG

References

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