thiM

168

hydroxyethylthiazole kinase

Locus: BSU_38300

Molecular weight: 28.06 kDa

Isoelectric point: 5.39

Protein length: 272 aa

Gene length: 819 bp

Function: biosynthesis of thiamine

Product: hydroxyethylthiazole kinase

Essential: no

E.C.: 2.7.1.50

Synonyms: thiM, thiK, ipa-25d, ywbJ

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG2145 (Galperin et al., 2021)

Gene

Coordinates: 3,931,372  3,932,190

The protein

Catalyzed reaction/ biological activity

• 5-(2-hydroxyethyl)-4-methylthiazole + ATP --> 4-methyl-5-(2-phosphooxyethyl)-thiazole + ADP + H⁺ (according to UniProt)

Protein family

• Thz kinase family (single member, according to UniProt)

Structure

• 1C3Q (PDB)
• P39593 (AlphaFold)

Expression and Regulation

Operons

• Genes: ywbI-thiM-thiE

  Description: PubMed

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE38300 (thiM::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTGCATCATCCTTTA,  downstream forward: _UP4_TCATGACTCGTATTTCTCGG
• BKK38300 (thiM::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATTTGCATCATCCTTTA,  downstream forward: _UP4_TCATGACTCGTATTTCTCGG

References

Reviews

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Original Publications

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