gcvPA

168

glycine decarboxylase (subunit 1)

Locus: BSU_24560

Molecular weight: 49.33 kDa

Isoelectric point: 6.95

Protein length: 448 aa

Gene length: 1347 bp

Function: glycine utilization

Product: glycine decarboxylase (subunit 1)

Essential: no

E.C.: 1.4.4.2

Synonyms: gcvPA, yqhJ, gcvP

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0403 (Galperin et al., 2021)

Gene

Coordinates: 2,546,869 → 2,548,215

The protein

Catalyzed reaction/ biological activity

• glycine + H⁺ + N⁶-lipoyl-L-lysyl-[glycine-cleavage complex H protein] --> (R)-N⁶-(S⁸-aminomethyldihydrolipoyl)-L-lysyl-[glycine-cleavage complex H protein] + CO₂ (according to UniProt)

Protein family

• GcvP family (with GcvPB, according to UniProt)

Structure

• 1WYT (PDB) (the GcvPA-GcvPB complex from Thermus thermophilus, 56% identity) PubMed
• P54376 (AlphaFold)

Expression and Regulation

Operons

• Genes: gcvT-gcvPA-gcvPB

  Description: PubMed

  Regulation

  • induced by glycine PubMed
  • the Gly-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • Gly-box: RNA switch, PubMed, in Gly-box

Biological materials

Mutant

• MGNA-C415 (yqhJ::erm), available at the NBRP B. subtilis, Japan
• BKE24560 (ΔgcvPA::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGTTGTCTCCTCTCTC,  downstream forward: _UP4_CTCATTCAGGAATTGGGGGA
• BKK24560 (ΔgcvPA::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGTGTTGTCTCCTCTCTC,  downstream forward: _UP4_CTCATTCAGGAATTGGGGGA

References

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