aroF

168

chorismate synthase

Locus: BSU_22710

Molecular weight: 40.04 kDa

Isoelectric point: 6.58

Protein length: 390 aa

Gene length: 1173 bp

Function: biosynthesis of aromatic amino acids

Product: chorismate synthase

Essential: yes

E.C.: 4.2.3.5

Synonyms: aroF

Genomic Context

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List of homologs in different organisms, belongs to COG0082 (Galperin et al., 2021)

Gene

Coordinates: 2,379,100  2,380,272

Phenotypes of a mutant

• essential PubMed

The protein

Catalyzed reaction/ biological activity

• 5-O-(1-carboxyvinyl)-3-phosphoshikimate --> chorismate + phosphate (according to UniProt)

Protein family

• chorismate synthase family (single member, according to UniProt)

Cofactors

• FAD (according to UniProt)

Structure

• 2O11 (PDB) (from Mycobacterium tuberculosis h37rv mutant, 42% identity, 58% similarity)
• P31104 (AlphaFold)

Modification

• phosphorylated on Arg-127 PubMed

Localization

• cytoplasm (according to Swiss-Prot)

Expression and Regulation

Operons

• Genes: cheR-aroF-aroB-aroH

  Description: PubMed

• Genes: aroF-aroB-aroH

  Description: PubMed

Biological materials

Mutant

• BKE22710 (aroF::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATGACTTCTTCTCCCTTCA,  downstream forward: _UP4_AGAAAACTGTCGAGGGAATT

References

Reviews

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Original Publications

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