Difference between revisions of "PGP380"

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[[File:PGP380.jpeg|250px|thumb|right|'''pGP380: click to enlarge''']]
 
[[File:PGP380.jpeg|250px|thumb|right|'''pGP380: click to enlarge''']]
 
[[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']]
 
[[File:PGP380_cloning_region.jpeg|250px|thumb|right|'''pGP380 cloning region: click to enlarge''']]
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'''Application:'''
  
Vector for the overexpression of N-terminally Strep-tagged proteins in ''B. subtilis'', used for the SPINE technology
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The vector was constructed in the [[Stülke]] lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in ''B. subtilis''. The plasmid confers resistance to ampicillin and erythromycin in ''E. coli'' and ''B. subtilis'', respectively. pGP380 can be used for the [[SPINE]] method. [[pGP382]] is similar to the vector pGP380 which is derived from vectors [[pBQ200]] and [[pHT315]].
  
constructed in [[Stülke]] lab
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Sequencing primers:  
 
 
in ''E. coli'': ampicillin resistance
 
 
 
in B''. subtilis'': erythromycin resistance
 
 
 
based on [[pBQ200]]
 
 
 
similar vector: [[pGP382]]
 
 
 
sequencing primer:  
 
 
*'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘
 
*'''M13_puc_for:''' 5‘-GTAAAACGACGGCCAGTG-3‘
 
*'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘
 
*'''M13_puc_rev:''' 5‘-GGAAACAGCTATGACCATG-3‘
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*'''FX125 (rev; primes -73bp of MCS):''' 5‘-GGCTCGTATGTTGTGTGG-3‘
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*'''JN54 (fwd; primes +148bp of MCS):''' 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘
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<pubmed> 17994626 </pubmed>
  
Herzberg, C., Flórez Weidinger, L. A., Dörrbecker, B., Hübner, S., Stülke, J. & Commichau, F. M. (2007) SPINE: A method for the rapid detection and analysis of protein-protein interactions in vivo. Proteomics 7: 4032-4035. [http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Abstract&list_uids=+17994626 PubMed]
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== Return to [[Plasmids]] ==

Latest revision as of 08:13, 29 July 2014

pGP380: click to enlarge
pGP380 cloning region: click to enlarge

Application:

The vector was constructed in the Stülke lab and it is suitable for constitutive overexpression of N-terminally Strep-tagged proteins in B. subtilis. The plasmid confers resistance to ampicillin and erythromycin in E. coli and B. subtilis, respectively. pGP380 can be used for the SPINE method. pGP382 is similar to the vector pGP380 which is derived from vectors pBQ200 and pHT315.

Sequencing primers:

  • M13_puc_for: 5‘-GTAAAACGACGGCCAGTG-3‘
  • M13_puc_rev: 5‘-GGAAACAGCTATGACCATG-3‘
  • FX125 (rev; primes -73bp of MCS): 5‘-GGCTCGTATGTTGTGTGG-3‘
  • JN54 (fwd; primes +148bp of MCS): 5‘-GTGAAAAATGAGCCGAAAGCAG-3‘


Christina Herzberg, Lope Andrés Flórez Weidinger, Bastian Dörrbecker, Sebastian Hübner, Jörg Stülke, Fabian M Commichau
SPINE: a method for the rapid detection and analysis of protein-protein interactions in vivo.
Proteomics: 2007, 7(22);4032-5
[PubMed:17994626] [WorldCat.org] [DOI] (P p)


Return to Plasmids