Difference between revisions of "PWH844"
Raphael2215 (talk | contribs) |
|||
| (8 intermediate revisions by 3 users not shown) | |||
| Line 1: | Line 1: | ||
[[File:PWH844.jpeg|250px|thumb|right|'''pWH844: click to enlarge''']] | [[File:PWH844.jpeg|250px|thumb|right|'''pWH844: click to enlarge''']] | ||
| + | [[File:PWH844_cloning_region.jpeg|250px|thumb|right|'''pWH844 cloning region: click to enlarge''']] | ||
| − | |||
| − | vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein | + | vector for the expression of proteins in ''E. coli'', the [[plasmid]] allows to fuse a His(6) tag to the N-terminus of the expressed protein |
| − | the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture | + | the [[plasmid]] carries the ''lacI'' gene, regulation is possible in any ''E. coli'' strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture |
constructed in the [[Hillen]] lab | constructed in the [[Hillen]] lab | ||
| − | in E. coli: ampicillin resistance | + | in ''E. coli'': ampicillin resistance |
| − | similar plasmid: [[pGP570]] | + | similar [[plasmids|plasmid]]: [[pGP570]] |
| − | + | sequencing primer: | |
| + | *<font color="#ff0000">'''JS39'''</font>: 5’-TCTATCAACAGGAGTCCAAGC-3‘ | ||
| + | *'''SH71:''' 5‘-'''AACGGTGGTA'''TATCCAGTG-3‘ | ||
| + | *'''PQE9-R348:''' 5’-TTGGGATATATC'''AACGGTGGTA'''-3’ | ||
| + | *<font color="#99E500">'''JK37'''</font>: 5’-GGATAACAATTATAATAGATTCAATTGTGAGCGGATAAC-3‘ | ||
| + | |||
| + | <pubmed> 9023219 </pubmed> | ||
| + | == Return to [[Plasmids]] == | ||
Latest revision as of 14:23, 8 October 2013
vector for the expression of proteins in E. coli, the plasmid allows to fuse a His(6) tag to the N-terminus of the expressed protein
the plasmid carries the lacI gene, regulation is possible in any E. coli strain, expression of the desired protein is induced by the addition of 1 mM IPTG to the culture
constructed in the Hillen lab
in E. coli: ampicillin resistance
sequencing primer:
- JS39: 5’-TCTATCAACAGGAGTCCAAGC-3‘
- SH71: 5‘-AACGGTGGTATATCCAGTG-3‘
- PQE9-R348: 5’-TTGGGATATATCAACGGTGGTA-3’
- JK37: 5’-GGATAACAATTATAATAGATTCAATTGTGAGCGGATAAC-3‘
F Schirmer, S Ehrt, W Hillen
Expression, inducer spectrum, domain structure, and function of MopR, the regulator of phenol degradation in Acinetobacter calcoaceticus NCIB8250.
J Bacteriol: 1997, 179(4);1329-36
[PubMed:9023219]
[WorldCat.org]
[DOI]
(P p)
