floT

168

membrane-associated scaffold protein, reduces membrane order, orchestration of physiological processes in lipid microdomains, involved in the control of membrane fluidity, confers (together with YuaF) resistance to cefuroxime

Locus: BSU_31010

Molecular weight: 55.82 kDa

Isoelectric point: 5.14

Protein length: 509 aa

Gene length: 1530 bp

Function: control of membrane fluidity

Product: membrane-associated scaffold protein

Essential: no

Synonyms: floT, yuaG, yuaH

Genomic Context

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List of homologs in different organisms, belongs to COG2268 (Galperin et al., 2021)

Gene

Coordinates: 3,180,465  3,181,994

Phenotypes of a mutant

• delayed onset of sporulation, reduced sporulation frequency
• defect in motility PubMed
• reduced protein secretion PubMed
• a ''floT floA'' double mutant does not induce KinC-dependent biofilm formation upon addition of surfactin PubMed
• a ''floT floA'' double mutant has a strong synthetic defect in motility, cell morphology, and transformation efficiency PubMed
• a ''floT floA'' double mutant has a sporulation defect, due to the lack of FtsH PubMed
• a ''floT'' mutant displays a defective growth under oxygen-limiting conditions PubMed

The protein

Catalyzed reaction/ biological activity

• SigW-dependent expression of ''fabF and the yuaF-floT-yuaI'' operon result in reduced membrane fluidity PubMed
• recruits YuaF to focal assemblies  PubMed
• controls protease activity of FtsH PubMed

Protein family

• Stomatin, Prohibitin, Flotillin, and HflK/C (SPFH) domain protein family (with YdjI and FloA) PubMed

Domains

• contains a SPFH domain (Stomatin, Prohibitin, Flotillin, and HflK/C-Domain) (aa 24 - 212) PubMed

Structure

• O32076 (AlphaFold)

Localization

• membrane associated PubMed
• co-localizes with FloA and KinC in discrete foci membrane PubMed
• forms discrete focal structures in the cytoplasma membrane PubMed
• 6 foci FloT of are detected in strain 3610 PubMed

Expression and Regulation

Operons

• Genes: yuaF-floT-yuaI

  Description: PubMed

  Regulation

  • expressed upon cell wall stress (SigW) PubMed

  Sigma factors

  • SigW: sigma factor, PubMed, in sigW regulon

Biological materials

Mutant

• MGNA-A213 (yuaG::erm), available at the NBRP B. subtilis, Japan
• JS152 (markerless), available in Daniel Lopez's lab
• BKE31010 (floT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCAAATTCCTCCTTTT,  downstream forward: _UP4_GAGTAAGGAAAGGGCAGAAC
• BKK31010 (floT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATCAAATTCCTCCTTTT,  downstream forward: _UP4_GAGTAAGGAAAGGGCAGAAC

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH) for FloT PubMed, available in Daniel Lopez's lab

GFP fusion

• GK38 (168 amyE::PfloT-yfp (spc)), available in Daniel Lopez's lab
• JS280 (3610 amyE::floT-gfp(spc)), available in Daniel Lopez's lab
• JS153 (3610 lacA::floT-mEos2 (mls)), available in Daniel Lopez's lab
• JS166 (3610 lacA::floT-PAmCherry (mls)), available in Daniel Lopez's lab

References

Reviews

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Original Publications

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