ricA

168

subunit of the regulatory iron-sulfur containing RicA-RicF-RicT complex, required for handing over of RicT to RNase Y to facilitate maturation of polycistronic mRNAs, antagonist of biofilm repression by SinR, control of the phosphorelay

Locus: BSU_17020

Molecular weight: 16.02 kDa

Isoelectric point: 5.09

Protein length: 143 aa

Gene length: 432 bp

Function: control of RNase Y activity, see Targets of the Y complex

Product: subunit of the regulatory iron-sulfur containing RicA-RicF-RicT complex

Essential: no

Synonyms: ymcA

Genomic Context

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List of homologs in different organisms, belongs to COG4550 (Galperin et al., 2021)

Gene

Coordinates: 1,774,374 → 1,774,805

Phenotypes of a mutant

• strongly reduced genetic competence, strongly reduced sporulation PubMed
• defective in RNase Y-mediated maturation of polycistronic mRNAs, see Targets of the Y complex PubMed
• defective in Swarming motility (prolonged period of immotility prior to Swarming) PubMed

The protein

Catalyzed reaction/ biological activity

• the RicA-RicF-RicT complex acts as specificity factor for RNase Y-dependent processing of polycistronic mRNAs, see Targets of the Y complex PubMed
• the RicA-RicF-RicT complex stimulates Spo0A phosphorylation in the phosphorelay PubMed

Cofactors

• one 4Fe-4S cluster (contributes to the co-ordination of the cluster (with RicF and RicT)) PubMed

Structure

• 6PRH (PDB) (the RicAdimer) PubMed
• 6PRK (PDB) (the RicA-RicF complex) PubMed
• O31779 (AlphaFold)

Localization

• cytoplasm PubMed

Expression and Regulation

Operons

• Genes: ymcB-ricA

  Description: PubMed

Biological materials

Mutant

• BKE17020 (ΔricA::erm  trpC2) available at BGSC and in Jörg Stülke's lab, PubMed, upstream reverse: _UP1_GTCTTTTTTTGAGTAGAGCG,  downstream forward: _UP4_TAAACACGGTGCCTTTACAG
• BKK17020 (ΔricA::kan  trpC2) available at BGSC, PubMed, upstream reverse: _UP1_GTCTTTTTTTGAGTAGAGCG,  downstream forward: _UP4_TAAACACGGTGCCTTTACAG

References

Reviews

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Original Publications

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