ykoT

168

similar to lipoteichoic acid glycosyltransferase

Locus: BSU_13390

Molecular weight: 38.36 kDa

Isoelectric point: 9.34

Protein length: 337 aa

Gene length: 1014 bp

Function: unknown

Product: unknown

Essential: no

Synonyms: ykoT

Genomic Context

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Categories containing this gene/protein

Lifestyles, Sporulation, Sporulation proteins, Newly identified sporulation proteins (based on transcription profiling)

Groups of genes, Membrane proteins

Groups of genes, Poorly characterized/ putative enzymes

List of homologs in different organisms, belongs to COG0463 (Galperin et al., 2021)

This gene is a member of the following regulons

SigG regulon, SpoVT regulon

Gene

Coordinates: 1,403,479 → 1,404,492

The protein

Protein family

glycosyltransferase 2 family (according to UniProt)

Structure

5EKP (PDB) (from Synechocystis sp., 38% identity) PubMed
O34755 (AlphaFold)

Paralogous protein(s)

CsbB
YkcC, (39%)

Localization

cell membrane (heterogeneous) PubMed

Expression and Regulation

Operons

Genes: ykoS-ykoT

Description: PubMed

Regulation

expressed late during sporulation in the forespore (SigG, SpoVT) PubMed

Regulatory mechanism

SpoVT: repression, PubMed, in spoVT regulon

Sigma factors

SigG: sigma factor, PubMed, in sigG regulon

Biological materials

Mutant

MGNA-A777 (ykoT::erm), available at the NBRP B. subtilis, Japan
BKE13390 (ΔykoT::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGACTGTTTCAATCGATATC,  downstream forward: _UP4_TAAACACGGAAAGAGCTGAC
BKK13390 (ΔykoT::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_TGACTGTTTCAATCGATATC,  downstream forward: _UP4_TAAACACGGAAAGAGCTGAC

References

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