yhbI

168

similar to transcriptional regulator ([wiki|MarR family])

locus: BSU_08990

Molecular weight: 17.46 kDa

pI: 8.69

Protein length: 154 aa

Gene length: 465 bp

function: unknown

product: unknown

essential: no

synonyms: yhbI

List of homologs in different organisms, belongs to COG1846 (Galperin et al., 2021)

Gene

Coordinates: 976,569 → 977,033

The protein

Protein family

• [wiki|MarR family]

[wiki|Domains]

• [wiki|HTH marR-type domain] (aa 1-143) (according to UniProt)

Structure

• [PDB|1S3J] ([protein|795D70E8A4D750140D6B1AA4BFEBD25C2A389C85|mdtR], corresponds to aa 38 - 139 of YhbI, 27% identity)
• [AF|O31592]

Expression and Regulation

Operons

• genes: [gene|EDDD61CF725449E57C72F63D378F6CD1FAC49ADA|yhbI]-[gene|211C58A92BA2A06A34B1F73BDB68A0F581B942C2|yhbJ]-[gene|93159DC7D5C42CFA54F66F232D73F38957243AB7|yhcA]-[gene|7797B0B66922D51B9D142DD2E5857836E47F015B|yhcB]-[gene|95EED3A91483502CA78067421F678B1EDB965B18|yhcC]

  description: [Pubmed|22383849]

  additional information

  • the mRNA is substantially stabilized upon depletion of [protein|872CCB5A49C9000BD95E4B0472556D5F60F7D7A4|RNase Y] (the half-life of the mRNA increases from 4 to 41 min) [PubMed|21815947]

Biological materials

Mutant

• MGNA-B469 (yhbI::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1468 NBRP B. subtilis, Japan]
• BKE08990 (Δ[gene|EDDD61CF725449E57C72F63D378F6CD1FAC49ADA|yhbI]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACTCCTTTTCACCTCATT,  downstream forward: _UP4_TAAAAACTTCTCACATATTT
• BKK08990 (Δ[gene|EDDD61CF725449E57C72F63D378F6CD1FAC49ADA|yhbI]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08990 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAACTCCTTTTCACCTCATT,  downstream forward: _UP4_TAAAAACTTCTCACATATTT

References

• 21815947