ebrB

168

multidrug efflux transporter

locus: BSU_17290

Molecular weight: 12.19 kDa

pI: 6.93

Protein length: 117 aa

Gene length: 354 bp

function: multidrug resistance

product: multidrug efflux transporter

essential: no

synonyms: ebrB

List of homologs in different organisms, belongs to COG2076 (Galperin et al., 2021)

Gene

Coordinates: 1,864,691  1,865,044

The protein

Protein family

• paired small multidrug resistance  protein family ([wiki|PSMR family]) [Pubmed|17942072]
• [wiki|drug/metabolite transporter (DMT) superfamily] (according to UniProt)
• [wiki|Small multidrug resistance (SMR) (TC 2.A.7.1) family] (according to UniProt)

Structure

• [PDB|3B5D] (EmrE from E. coli, 39% identity) [pubmed|18024586]

Paralogous protein(s)

• [protein|53576746FF1B644A1980F8ACD0E8F4A54CDDAD83|ebrA]

[wiki|Localization]

• cell membrane [Pubmed|17417881]

Expression and Regulation

Operons

• genes: [gene|53576746FF1B644A1980F8ACD0E8F4A54CDDAD83|ebrA]-[gene|74668FF19D097722D523CC54288B6409DE267631|ebrB]

  description: [Pubmed|22383849]

Biological materials

Mutant

• MGNA-B077 (ebrB::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1076 NBRP B. subtilis, Japan]
• BKE17290 ([gene|74668FF19D097722D523CC54288B6409DE267631|ebrB]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE17290 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATCCTCTCATCTTCATCA,  downstream forward: _UP4_GCCTGTGAGTGACCGTCTGT
• BKK17290 ([gene|74668FF19D097722D523CC54288B6409DE267631|ebrB]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK17290 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CAATCCTCTCATCTTCATCA,  downstream forward: _UP4_GCCTGTGAGTGACCGTCTGT

References

Reviews

• 17942072

Original Publications

• 17417881,18024586