metI

168

O-succinylhomoserine lyase (L-cysteine, H₂S, methanethiol, elimination)

Locus: BSU_11870

Molecular weight: 41.55 kDa

Isoelectric point: 5.02

Protein length: 373 aa

Gene length: 1119 bp

Function: biosynthesis of methionine

Product: O-succinylhomoserine lyase (L-cysteine, H₂S, methanethiol, elimination)

Essential: no

Synonyms: metI, yjcI

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0626 (Galperin et al., 2021)

Gene

Coordinates: 1,258,492 → 1,259,613

The protein

Catalyzed reaction/ biological activity

• L-cysteine + O-acetyl-L-homoserine --> acetate + H⁺ + L,L-cystathionine (according to UniProt)
• hydrogen sulfide + O-acetyl-L-homoserine --> acetate + L-homocysteine (according to UniProt)

Protein family

• trans-sulfuration enzymes family (with MccB and MetC, according to UniProt)

Cofactors

• PLP (according to UniProt)

Structure

• 4L0O (PDB) (from Helicobacter pylori, 48% identity)
• O31631 (AlphaFold)

Paralogous protein(s)

• MetC, MccB

Expression and Regulation

Operons

• Genes: metI-metC

  Description: PubMed

  Regulation

  • induced by methionine starvation (S-box) PubMed
  • the S-box RNA is degraded by RNase Y PubMed

  Regulatory mechanism

  • S-box: RNA switch, the S-box riboswitch binds S-adenosylmethionine resulting in termination, in S-box

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B298 (yjcI::erm), available at the NBRP B. subtilis, Japan
• 1A941 ( metI::spec), PubMed, available at BGSC
• BKE11870 (ΔmetI::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAGCTGACACCTTCCC,  downstream forward: _UP4_CAGGTCAAAGAGGGAGCTGT
• BKK11870 (ΔmetI::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATATAGCTGACACCTTCCC,  downstream forward: _UP4_CAGGTCAAAGAGGGAGCTGT

References

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