yqiD

168

geranyltransferase

Locus: BSU_24280

Molecular weight: 29.59 kDa

Isoelectric point: 5.02

Protein length: 296 aa

Gene length: 891 bp

Function: biosynthesis of peptidoglycan and teichoic acid

Product: geranyltransferase

Essential: no

E.C.: 2.5.1.10

Synonyms: yqiD, ispA

Genomic Context

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List of homologs in different organisms, belongs to COG0142 (Galperin et al., 2021)

Gene

Coordinates: 2,525,789 → 2,526,679

Phenotypes of a mutant

• The mutation of yqiD predisposes cells to grow without a wall PubMed

The protein

Catalyzed reaction/ biological activity

• (2E)-geranyl diphosphate + isopentenyl diphosphate --> (2E,6E)-farnesyl diphosphate + pp_i (according to UniProt)

Protein family

• FPP/GGPP synthase family (with HepT, according to UniProt)

Cofactors

• Magnesium

Structure

• 1RQI (PDB): IspA from E. coli.  Co-crystal with substrate isopentyl pyrophosphate and substrate analogue dimethyallyl S-thiolodiphosphate PubMed
• P54383 (AlphaFold)

Modification

• None

Paralogous protein(s)

• HepT

Biological materials

Mutant

• BKE24280 (ΔyqiD::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCGGTCCGCCAGAAAGCTCG,  downstream forward: _UP4_TAAAACGATCCTAATTTGAA
• BKK24280 (ΔyqiD::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CCGGTCCGCCAGAAAGCTCG,  downstream forward: _UP4_TAAAACGATCCTAATTTGAA

References

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