pksS

168

cytochrome P450, hydroxylase of the polyketide bacillaene produced by the pks cluster

Locus: BSU_17230

Molecular weight: 43.26 kDa

Isoelectric point: 6.12

Protein length: 405 aa

Gene length: 1218 bp

Function: polyketide synthesis

Product: hydroxylase of the polyketide produced by the pks cluster

Essential: no

Synonyms: pksS

Genomic Context

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List of homologs in different organisms, belongs to COG2124 (Galperin et al., 2021)

Gene

Coordinates: 1,858,566 → 1,859,783

The protein

Catalyzed reaction/ biological activity

• hydroxylation of dihydrobacillaene PubMed

Protein family

• cytochrome P450 family (according to UniProt)

Structure

• 4YZR (PDB)
• O31785 (AlphaFold)

Paralogous protein(s)

• CypA, BioI, YjiB

Expression and Regulation

Operons

• Genes: pksS

  Description: PubMed

Biological materials

Mutant

• BKE17230 (ΔpksS::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCATTCTCCTCGCCT,  downstream forward: _UP4_TAACATTCAAAACGCCCCCT
• BKK17230 (ΔpksS::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGCATTCTCCTCGCCT,  downstream forward: _UP4_TAACATTCAAAACGCCCCCT

References

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