ssuD

168

aliphatic sulfonate monooxygenase

locus: BSU_08860

Molecular weight: 41.64 kDa

pI: 5.37

Protein length: 376 aa

Gene length: 1131 bp

function: sulfonate reduction

product: aliphatic sulfonate monooxygenase

essential: no

ec: null

synonyms: ssuD, ygcA, yzeC

List of homologs in different organisms, belongs to COG2141 (Galperin et al., 2021)

Gene

Coordinates: 964,027  965,157

The protein

Catalyzed reaction/ biological activity

• alkanesulfonate + FMNH₂ + O₂ --> aldehyde + FMN + 2 H⁺ + H₂O + sulfite (according to UniProt)

Protein family

• ssuD family (single member, according to UniProt)

Structure

• [PDB|1NQK] (from ''Escherichia coli'', 64% identity, 76% similarity)
• [AF|P40402]

Expression and Regulation

Operons

• genes: [gene|675CE0BCA393E9C2795032F1C0FDE6AF08B77033|ssuB]-[gene|B47C64731FCAA086CA563EEE95D3205428E51EFC|ssuA]-[gene|91EAC386AD3A373E7CDE0265AA23786ADC55558A|ssuC]-[gene|A9F7BEC330EB17CA38C4D421E8BBF58CE380559C|ssuD]-[gene|93F7A5795A423CD8DD7B070F7F37731A5E8D6321|ygaN]

  description: [Pubmed|11251850]

  regulation

  • repressed in the presence of cysteine ([protein|search|CymR]) [Pubmed|16513748]

  regulatory mechanism

  • [protein|50930C56C27D22715620A350220E3C56ADB41020|cymR]: repression, [Pubmed|16513748], in [regulon|protein:50930C56C27D22715620A350220E3C56ADB41020|cymR regulon]

  sigma factors

  • [protein|360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA]: sigma factor, [Pubmed|11251850], in [regulon|protein:360F48D576DE950DF79C1A2677B7A35A8D8CC30C|sigA regulon]

Biological materials

Mutant

• MGNA-B466 (ygcA::erm), available at the [https://shigen.nig.ac.jp/bsub/resource/strainGeneDisrupted/detail/1465 NBRP B. subtilis, Japan]
• 1A951 ( ''ssuD''::''cat''), [Pubmed| ], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A951&Search=1A951 BGSC]
• 1A8083 ( ''ssuD''::''kan''), [Pubmed|11445163], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A8083&Search=1A8083 BGSC]
• BKE08860 ([gene|A9F7BEC330EB17CA38C4D421E8BBF58CE380559C|ssuD]::erm  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKE08860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCATCTCCCCTTTAT,  downstream forward: _UP4_TGAGTTTAACTAAAATACAG
• BKK08860 ([gene|A9F7BEC330EB17CA38C4D421E8BBF58CE380559C|ssuD]::kan  trpC2) available at [http://bgsc.org/getdetail.php?bgscid=BKK08860 BGSC],  [Pubmed|28189581], upstream reverse: _UP1_CATATTCATCTCCCCTTTAT,  downstream forward: _UP4_TGAGTTTAACTAAAATACAG

References

• 15937167,11251850,16513748,9782504