sigK

168

RNA polymerase sporulation-specific sigma factor (sigma-K) (5' part of the interrupted sigK gene), with sigKC

Locus: BSU_25760

Molecular weight: 17.00 kDa

Isoelectric point: 8

Protein length: 156 aa

Gene length: 471 bp

Function: late mother cell-specific gene expression

Product: RNA polymerase sporulation-specific sigma factor(sigma-K) (5' part of the gene)

Essential: no

Synonyms: sigK, sigKN, spoIVCB

Genomic Context

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List of homologs in different organisms, belongs to COG5814 (Galperin et al., 2021)

Gene

Coordinates: 2,652,993  2,653,463

The protein

Protein family

• Sigma-70 factor family (according to UniProt)

Structure

• 8VJL (PDB) (SpoIVFB-Pro-SigK) PubMed
• P12254 (AlphaFold)

Expression and Regulation

Operons

• Genes: sigK

  Description: (composed of spoIVCB-spoIIIC) PubMed

  Regulation

  • expressed during sporulation in the mother cell (SigE, SigK, GerE, SpoIIID) PubMed

  Regulatory mechanism

  • GerE: repression, PubMed, in gerE regulon
  • SpoIIID: activation, PubMed, in spoIIID regulon
  • ScoC: repression, PubMed, in scoC regulon

  Sigma factors

  • SigE: sigma factor, PubMed, in sigE regulon
  • SigK: sigma factor, PubMed, in sigK regulon

  Additional information

  • 'spoIVCB' and 'spoIIIC' form a composite gene in the mother cell during sporulation by excising the intervening DNA sequence.
  • the mRNA is degraded by KapD PubMed

Biological materials

Mutant

• BKE25760 (sigK::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTCACCTCCACAAAAG,  downstream forward: _UP4_AAAGGGGGGTGCATACACCC
• BKK25760 (sigK::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATCGTCACCTCCACAAAAG,  downstream forward: _UP4_AAAGGGGGGTGCATACACCC

References

Reviews

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Original Publications

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The SigK regulon

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Labs working on this gene/protein

• Arthur Aronson, Purdue University, West Lafayette, USA homepage
• Charles Moran, Emory University, NC, USA homepage