hom

168

homoserine dehydrogenase (NADPH)

Locus: BSU_32260

Molecular weight: 47.33 kDa

Isoelectric point: 4.9

Protein length: 433 aa

Gene length: 1302 bp

Function: biosynthesis of methionine and threonine

Product: homoserine dehydrogenase (NADPH)

Essential: no

E.C.: 1.1.1.3

Synonyms: hom

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG0460 (Galperin et al., 2021)

Gene

Coordinates: 3,314,828 3,316,129

The protein

Catalyzed reaction/ biological activity

• L-aspartate 4-semialdehyde + NAD(P)H+H⁺ --> L-homoserine + NAD(P)⁺ PubMed

Protein family

• Homoserine dehydrogenase family (single member, according to UniProt)

Domains

• ACT domain (aa 350-426) (according to UniProt)

Cofactors

• NADPH PubMed

Structure

• 2EJW (PDB) (from Thermus thermophilus hb8, 37% identity, 57% similarity)
• P19582 (AlphaFold)

Additional information

• belongs to the 100 most abundant proteins PubMed

Expression and Regulation

Operons

• Genes: yutH-hom-thrC-thrB

  Description: PubMed

  Regulation

  • expressed during sporulation PubMed

• Genes: hom-thrC-thrB

  Description: PubMed

  Regulation

  • expressed in the presence of lysine or cysteine (ThrR) PubMed

  Regulatory mechanism

  • CodY: repression, PubMed, in codY regulon
  • TnrA: repression, PubMed, in tnrA regulon
  • ThrR: repression, PubMed, in thrR regulon

Biological materials

Mutant

• BKE32260 (hom::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG
• BKK32260 (hom::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CAAAAAAACTCCACCTTTCT, downstream forward: _UP4_GTAGAAGGGAACGGTTGGAG

LacZ fusion

• pBP312 (in pAC5), available in Fabian Commichau's lab PubMed

References

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