gutB

168

D-sorbitol dehydrogenase

Locus: BSU_06150

Molecular weight: 38.23 kDa

Isoelectric point: 5.21

Protein length: 353 aa

Gene length: 1062 bp

Function: glucitol utilization

Product: D-sorbitol dehydrogenase

Essential: no

E.C.: 1.1.1.14

Synonyms: gutB

Genomic Context

Hide small RNAsZoom:

List of homologs in different organisms, belongs to COG1063 (Galperin et al., 2021)

Gene

Coordinates: 667,466  668,527

The protein

Catalyzed reaction/ biological activity

• D-fructose + H⁺ + NADH --> D-sorbitol + NAD⁺ (according to UniProt)
• NAD⁺ + xylitol --> D-xylulose + H⁺ + NADH (according to UniProt)
• L-iditol + NAD⁺ --> H⁺ + L-sorbose + NADH (according to UniProt)

Protein family

• zinc-containing alcohol dehydrogenase family (according to UniProt)

Cofactors

• NAD⁺ (according to UniProt)

Structure

• 1PL7 (PDB) (the human enzyme, 42% identity) PubMed
• Q06004 (AlphaFold)

Expression and Regulation

Operons

• Genes: gutB-gutP-fruC

  Description: PubMed

  Regulation

  • induced by glucitol (GutR) PubMed

  Regulatory mechanism

  • GutR: activation, PubMed, in gutR regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• BKE06150 (gutB::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGGCAAGTTCCTTTCT,  downstream forward: _UP4_TGAGTGAACAGGGAGGATCT
• BKK06150 (gutB::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTGGCAAGTTCCTTTCT,  downstream forward: _UP4_TGAGTGAACAGGGAGGATCT

References

Loading