ytpP

168

cleavage of CoA moieties from modified proteins, similar to thioredoxin H1

Locus: BSU_29840

Molecular weight: 12.61 kDa

Isoelectric point: 4.3

Protein length: 107 aa

Gene length: 324 bp

Function: cleavage of CoA moieties from modified proteins

Product: protein deCoAlase

Essential: no

Synonyms: ytpP

Genomic Context

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List of homologs in different organisms, belongs to COG3118 (Galperin et al., 2021)

Gene

Coordinates: 3,054,188  3,054,511

The protein

Catalyzed reaction/ biological activity

• cleavage of CoA moieties from modified proteins PubMed

Domains

• Thioredoxin domain (aa 1-105) (according to UniProt)

Structure

• 4RUV (PDB) (Thioredoxin-2 from Staphylococcus aureus, 32% identity)
• O34357 (AlphaFold)

Expression and Regulation

Operons

• Genes: ytpP-ytpQ-ytpR

  Description: PubMed

  Regulatory mechanism

  • Spx: activation, PubMed, in spx regulon

  Sigma factors

  • SigA: sigma factor, PubMed, in sigA regulon

Biological materials

Mutant

• MGNA-B531 (ytpP::erm), available at the NBRP B. subtilis, Japan
• BKE29840 (ytpP::erm  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCATATCCCTCCAGTT,  downstream forward: _UP4_AAAGCGTAAAGGAGGACATC
• BKK29840 (ytpP::kan  trpC2) available at BGSC,  PubMed, upstream reverse: _UP1_CATTTCATATCCCTCCAGTT,  downstream forward: _UP4_AAAGCGTAAAGGAGGACATC

References

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